Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kar, Gozde | - |
dc.contributor.author | Kim, Jong Kyoung | - |
dc.contributor.author | Kolodziejczyk, Aleksandra A. | - |
dc.contributor.author | Natarajan, Kedar Nath | - |
dc.contributor.author | Triglia, Elena Torlai | - |
dc.contributor.author | Mifsud, Borbala | - |
dc.contributor.author | Elderkin, Sarah | - |
dc.contributor.author | Marioni, John C. | - |
dc.contributor.author | Pombo, Ana | - |
dc.contributor.author | Teichmann, Sarah A. | - |
dc.date.available | 2017-08-10T08:10:43Z | - |
dc.date.created | 2017-08-09 | - |
dc.date.issued | 2017-06 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/4143 | - |
dc.description.abstract | Polycomb repressive complexes (PRCs) are important histone modifiers, which silence gene expression; yet, there exists a subset of PRC-bound genes actively transcribed by RNA polymerase II (RNAPII). It is likely that the role of Polycomb repressive complex is to dampen expression of these PRC-active genes. However, it is unclear how this flipping between chromatin states alters the kinetics of transcription. Here, we integrate histone modifications and RNAPII states derived from bulk ChIP-seq data with single-cell RNA-sequencing data. We find that Polycomb repressive complex-active genes have greater cell-to-cell variation in expression than active genes, and these results are validated by knockout experiments. We also show that PRC-active genes are clustered on chromosomes in both two and three dimensions, and interactions with active enhancers promote a stabilization of gene expression noise. These findings provide new insights into how chromatin regulation modulates stochastic gene expression and transcriptional bursting, with implications for regulation of pluripotency and development. © 2017 The Author(s). | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/s41467-017-00052-2 | - |
dc.identifier.scopusid | 2-s2.0-85021663714 | - |
dc.identifier.bibliographicCitation | Nature Communications, v.8, no.1 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordPlus | Allele | - |
dc.subject.keywordPlus | Animal Cell | - |
dc.citation.number | 1 | - |
dc.citation.title | Nature Communications | - |
dc.citation.volume | 8 | - |