The study on the effects of vutiglabridin in the gastrocnemius muscle of aged mice
- Title
- The study on the effects of vutiglabridin in the gastrocnemius muscle of aged mice
- Alternative Title
- Vutiglabridin이 노화 마우스의 비장근에 미치는 효과에 대한 연구
- Author(s)
- Jihan Lee
- DGIST Authors
- Jihan Lee ; Chang-Hoon Nam ; Young-Sam Lee
- Advisor
- 남창훈
- Co-Advisor(s)
- Young-Sam Lee
- Issued Date
- 2023
- Awarded Date
- 2023-02-01
- Type
- Thesis
- Description
- Aging, Vutiglabridin, Satellite cell, GA muscle, ROS
- Table Of Contents
-
Ⅰ. Introduction 1
Ⅱ. Experimental Method & Materials
2.1. Mice 6
2.2. RNA extraction 6
2.3. Real-time polymerase chain reaction (RT-qPCR) 7
2.4. Bulk RNA sequencing 7
2.5. Senescence-associated β-galactosidase (SA-β-gal) staining 8
2.6. Glutathione assay 8
2.7. Statistics 8
Ⅲ. Result
3.1. Vutiglabridin tended to decrease the senescence-related gene expressions in the GA muscle of aged mice 10
3.2. Vutiglabridin could affect ECM, ROS, mitochondria, and RNA processing gene ontology pathways and age-associated lncRNA in the GA muscle of aged mice 11
3.3. Vutiglabridin may modulate satellite cell function-related gene expressions in the GA muscle of aged mice 12
3.4. Vutiglabridin may change alternative splicing-related gene expressions in the GA muscle of aged mice 16
3.5. Vutiglabridin regulated the senescence-related gene expressions in the spleen and kidney of aged mice 17
3.6. Vutiglabridin may reduce the SA-β-gal activity but vutiglabridin did not affect the ratio of glutathione in the spleen and kidney of aged mice 18
Ⅳ. Discussion 20
Ⅴ. Conclusion 25
Ⅵ. Figures 26
Ⅶ. References 38
- URI
-
http://hdl.handle.net/20.500.11750/45707
http://dgist.dcollection.net/common/orgView/200000655565
- Degree
- Master
- Department
- Department of New Biology
- Publisher
- DGIST
- Related Researcher
-
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Nam, Chang-Hoon
- Research Interests Protein Engineering; History and Philosophy of Science; Scientific Art; Biomaterial Development (Tissue Engineering) with modified bacteriophage; Bio Sensor Development
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- Appears in Collections:
- Department of New Biology Theses Master
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