Title

The study on the effects of vutiglabridin in the gastrocnemius muscle of aged mice

Alternative Title

Vutiglabridin이 노화 마우스의 비장근에 미치는 효과에 대한 연구

DGIST Authors

Jihan Lee ; Chang-Hoon Nam ; Young-Sam Lee

Advisor

남창훈

Co-Advisor(s)

Young-Sam Lee

Issued Date

2023

Awarded Date

2023-02-01

Citation

Jihan Lee. (2023). The study on the effects of vutiglabridin in the gastrocnemius muscle of aged mice. doi: 10.22677/THESIS.200000655565

Type

Thesis

Description

Aging, Vutiglabridin, Satellite cell, GA muscle, ROS

Table Of Contents

Ⅰ. Introduction 1

Ⅱ. Experimental Method & Materials
2.1. Mice 6
2.2. RNA extraction 6
2.3. Real-time polymerase chain reaction (RT-qPCR) 7
2.4. Bulk RNA sequencing 7
2.5. Senescence-associated β-galactosidase (SA-β-gal) staining 8
2.6. Glutathione assay 8
2.7. Statistics 8

Ⅲ. Result
3.1. Vutiglabridin tended to decrease the senescence-related gene expressions in the GA muscle of aged mice 10
3.2. Vutiglabridin could affect ECM, ROS, mitochondria, and RNA processing gene ontology pathways and age-associated lncRNA in the GA muscle of aged mice 11
3.3. Vutiglabridin may modulate satellite cell function-related gene expressions in the GA muscle of aged mice 12
3.4. Vutiglabridin may change alternative splicing-related gene expressions in the GA muscle of aged mice 16
3.5. Vutiglabridin regulated the senescence-related gene expressions in the spleen and kidney of aged mice 17
3.6. Vutiglabridin may reduce the SA-β-gal activity but vutiglabridin did not affect the ratio of glutathione in the spleen and kidney of aged mice 18

Ⅳ. Discussion 20

Ⅴ. Conclusion 25

Ⅵ. Figures 26

Ⅶ. References 38

URI

http://hdl.handle.net/20.500.11750/45707
http://dgist.dcollection.net/common/orgView/200000655565

DOI

10.22677/THESIS.200000655565

Degree

Master

Department

Department of New Biology

Publisher

DGIST

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