DGIST Scholar: The study on the effects of vutiglabridin in the gastrocnemius muscle of aged mice

Title: The study on the effects of vutiglabridin in the gastrocnemius muscle of aged mice

Citation: Jihan Lee. (2023). The study on the effects of vutiglabridin in the gastrocnemius muscle of aged mice. doi: 10.22677/THESIS.200000655565

Table Of Contents: Ⅰ. Introduction 1

Ⅱ. Experimental Method & Materials
2.1. Mice 6
2.2. RNA extraction 6
2.3. Real-time polymerase chain reaction (RT-qPCR) 7
2.4. Bulk RNA sequencing 7
2.5. Senescence-associated β-galactosidase (SA-β-gal) staining 8
2.6. Glutathione assay 8
2.7. Statistics 8

Ⅲ. Result
3.1. Vutiglabridin tended to decrease the senescence-related gene expressions in the GA muscle of aged mice 10
3.2. Vutiglabridin could affect ECM, ROS, mitochondria, and RNA processing gene ontology pathways and age-associated lncRNA in the GA muscle of aged mice 11
3.3. Vutiglabridin may modulate satellite cell function-related gene expressions in the GA muscle of aged mice 12
3.4. Vutiglabridin may change alternative splicing-related gene expressions in the GA muscle of aged mice 16
3.5. Vutiglabridin regulated the senescence-related gene expressions in the spleen and kidney of aged mice 17
3.6. Vutiglabridin may reduce the SA-β-gal activity but vutiglabridin did not affect the ratio of glutathione in the spleen and kidney of aged mice 18

Ⅳ. Discussion 20

Ⅴ. Conclusion 25

Ⅵ. Figures 26

Ⅶ. References 38

URI: http://hdl.handle.net/20.500.11750/45707
http://dgist.dcollection.net/common/orgView/200000655565

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