Method establishment for mass spectrometry-based proteomics to identify lung cancer biomarkers from bronchoalveolar lavage fluid
- Title
- Method establishment for mass spectrometry-based proteomics to identify lung cancer biomarkers from bronchoalveolar lavage fluid
- Author(s)
- Minh Hung Vu
- DGIST Authors
- Minh Hung Vu ; Min-Sik Kim ; Seung Hyeun Lee
- Advisor
- 김민식
- Co-Advisor(s)
- Seung Hyeun Lee
- Issued Date
- 2023
- Awarded Date
- 2023-02-01
- Type
- Thesis
- Description
- lung cancer, proteomic, bronchoalveolar lavage fluid, biomarkers
- Table Of Contents
-
Chapter I: Introduction 1
1.1 Lung cancer 1
1.1.1 Non-small cell lung cancer 1
1.1.2 Small-cell lung cancer 2
1.2 Biomarker for lung cancer 3
1.3 Mass spectrometry-based proteomic analysis in biomarker discovery 5
1.3.1 Method in protein identification using mass spectrometry 5
1.3.2 Method in protein quantification using mass spectrometry 6
1.3.3 Challenges in biomarker identification by mass spectrometry-based proteomic analysis. 7
1.4 Quantitative proteomic analysis of BALF collected from lung cancer patients in this thesis 8
Chapter II: Parallel Accumulation- Serial Fragmentation method in proteomic analysis of BALF collected from NSCLC patients 9
2.1 Introduction 9
2.2 Results 12
2.2.1 Experimental design for evaluation of PASEF method in BALF proteomic analysis 12
2.2.2 PASEF increase sensitivity of BALF proteome profiling 12
2.2.3 Performance of PASEF in BALF quantitative analysis 13
2.3 Discussion 14
2.4 Materials and methods 15
Chapter III: Developing tipSCX method for proteomic analysis of BALF collected from SCLC patients 22
3.1 Introduction 22
3.2 Results 24
3.2.1 Development of tipSCX for BALF proteome analysis 24
3.2.2 Proteome profile of BALF collected from SCLC patients 25
3.2.3 Differentially expressed proteins in SCLC T-BALF 26
3.3 Discussion 28
3.4 Materials and methods 29
Chapter IV. Identification of potential biomarker for lung cancer from T-BALF 36
4.1 Introduction 36
4.2 Results 37
4.2.1 Identification of different expressed protein in between T-BALF and N-BALF from lung cancer patients 37
4.2.2 Identification of potential biomarker from T-BALF 38
4.2.3 Up-regulated proteins in T-BALF as potential indicators for prognosis 39
4.2.4 Up-regulated proteins in T-BALF as potential indicator for therapy response 40
4.3 Conclusion and discussion 41
4.4 Materials and methods 43
Chapter V. Conclusion 61
Reference 63
요약 70
- URI
-
http://hdl.handle.net/20.500.11750/45777
http://dgist.dcollection.net/common/orgView/200000655801
- Degree
- Doctor
- Department
- Department of New Biology
- Publisher
- DGIST
- Related Researcher
-
-
Kim, Min-Sik
- Research Interests Cancer Proteogenomics; Biomarker Discovery; Integrative Multi-Omics
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- Files in This Item:
-
There are no files associated with this item.
- Appears in Collections:
- Department of New Biology Theses Ph.D.
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