Title

Bioinspired Surface Biofunctionalization Technique for Immobilization of Biochemical Probes

Alternative Title

생화학적 프로브 고정화를 위한 생체 모사 표면 처리 기술

DGIST Authors

Chorok Byeon ; Seonki Hong ; Jinhee Park

Advisor

홍선기

Co-Advisor(s)

Jinhee Park

Issued Date

2023

Awarded Date

2023-08-01

Citation

Chorok Byeon. (2023). Bioinspired Surface Biofunctionalization Technique for Immobilization of Biochemical Probes. doi: 10.22677/THESIS.200000684527

Type

Thesis

Description

Surface treatment; Probe immobilization; Catecholamine; One-pot coating; 표면 처리; 프로브 고정화; 카테콜아민; 원 포트 코팅

Table Of Contents

I. Introduction 1
IⅠ. Experimental Section 3
2.1 Materials 3
2.2 Surface modification 4
2.3 Cell culture 4
2.4 Exosome isolation from cell culture media 5
2.5 Biotinylation 5
2.6 Detection of exosomes using PDA/neutravidin-coated substrates 6
2.7 Surface-independent immobilization of antibodies and detection of exosomes 6
III. Results and Discussion 8
3.1 Surface modification 8
3.1.1 Quantification and analysis of the coating surfaces 9
3.1.2 Scanning electron microscopy images of plate surfaces 11
3.2 Detection of exosomes using PDA/neutravidin-coated substrates 12
3.3 Surface-independent immobilization of antibodies and detection of exosomes 17
IV. Conclusion 20
References 21
요약문 23

URI

http://hdl.handle.net/20.500.11750/46454
http://dgist.dcollection.net/common/orgView/200000684527

DOI

10.22677/THESIS.200000684527

Degree

Master

Department

Department of Physics and Chemistry

Publisher

DGIST

Show Full Item Record