Bioinspired Surface Biofunctionalization Technique for Immobilization of Biochemical Probes
- Title
- Bioinspired Surface Biofunctionalization Technique for Immobilization of Biochemical Probes
- Alternative Title
- 생화학적 프로브 고정화를 위한 생체 모사 표면 처리 기술
- Author(s)
- Chorok Byeon
- DGIST Authors
- Chorok Byeon ; Seonki Hong ; Jinhee Park
- Advisor
- 홍선기
- Co-Advisor(s)
- Jinhee Park
- Issued Date
- 2023
- Awarded Date
- 2023-08-01
- Type
- Thesis
- Description
- Surface treatment; Probe immobilization; Catecholamine; One-pot coating; 표면 처리; 프로브 고정화; 카테콜아민; 원 포트 코팅
- Table Of Contents
-
I. Introduction 1
IⅠ. Experimental Section 3
2.1 Materials 3
2.2 Surface modification 4
2.3 Cell culture 4
2.4 Exosome isolation from cell culture media 5
2.5 Biotinylation 5
2.6 Detection of exosomes using PDA/neutravidin-coated substrates 6
2.7 Surface-independent immobilization of antibodies and detection of exosomes 6
III. Results and Discussion 8
3.1 Surface modification 8
3.1.1 Quantification and analysis of the coating surfaces 9
3.1.2 Scanning electron microscopy images of plate surfaces 11
3.2 Detection of exosomes using PDA/neutravidin-coated substrates 12
3.3 Surface-independent immobilization of antibodies and detection of exosomes 17
IV. Conclusion 20
References 21
요약문 23
- URI
-
http://hdl.handle.net/20.500.11750/46454
http://dgist.dcollection.net/common/orgView/200000684527
- Degree
- Master
- Department
- Department of Physics and Chemistry
- Publisher
- DGIST
- Related Researcher
-
-
Hong, Seonki
- Research Interests Bio-inspired organic materials; Polymeric biomaterials; Surface biofunctionalization; biochip fabrication
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- Files in This Item:
-
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- Appears in Collections:
- Department of Physics and Chemistry Theses Master
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