Title

Unveiling fat depot-specific responses upon metabolic stimuli through single-cell and spatial transcriptome analysis

DGIST Authors

Eun Seo Park ; June M. Kwak ; Jong Kyoung Kim

Advisor

곽준명

Co-Advisor(s)

Jong Kyoung Kim

Issued Date

2024

Awarded Date

2024-02-01

Citation

Eun Seo Park. (2024). Unveiling fat depot-specific responses upon metabolic stimuli through single-cell and spatial transcriptome analysis. doi: 10.22677/THESIS.200000730444

Type

Thesis

Description

single-cell RNA-seq;spatial transcriptome;white adipose tissue;brown adipose tissue;adipose stem cell

Table Of Contents

Ⅰ. Introduction
1.1 Adipose Tissue · 1
1.2 Adipose tissue upon metabolic stimuli · 1
1.3 Adipose tissue at single cell resolution · 2
1.4 Aims and objectives · 3
IⅠ. Materials and methods
2.1 Animals 4
2.2 Human Subjects 4
2.3 scRNA-seq Library Preparation 4
2.4 scRNA-seq Data Procession · 5
2.5 scRNA-seq Data Analysis 6
2.6 Adipose Tissue Fractionation 7
2.7 Fluorescence-Activated Cell Sorting (FACS) 7
2.8 Isolation of SVFs from Human Adipose Tissue 7
2.9 Immunocytochemistry and Immunohistochemistry · 8
2.10 Isolation of Adipocyte Nuclei · 8
2.11 Cell Culture 9
2.12 Proliferation Assay · 9
2.13 Monocyte Migration Assay · 10
2.14 siRNA Treatment · 10
2.15 RT-qPCR · 11
2.16 Transplantation and Lymph Node (LN) Dissection · 11
2.17 Statistical Analysis 11
2.18 Spatial-seq Data Analysis 12
ⅠII. Results
3.1 Under homeostatic conditions, ASC clusters in IAT and EAT display distinct
molecular properties and consist of three adipogenic stages 17
3.2 The adipogenic potential of ES1 and IS1 ASCs is primarily determined by their
intrinsic factors 27
3.3 In EAT, obesogenic stimuli enhance ES1 ASC proliferation through FGF and TGFβ
signaling, leading to de novo adipogenesis · 33
3.4 In obesity, EAT-specific SDC1+ regulate fibrosis and inflammation in a depot-
specific manner 39
3.5 During obesity, IAT specific CXCL14+ ASCs exert a suppressive effect on
monocyte infiltration 45
3.6 BST2high ASCs specific to IAT serve as precursors for beige adipocytes, and their
biogenesis is under the regulation of lymph nodes (LNs) 53
ⅠV. Discussion
Discussion · 65
Acknowledgement · 68
References · 69
요약문 79

URI

http://hdl.handle.net/20.500.11750/48004
http://dgist.dcollection.net/common/orgView/200000730444

DOI

10.22677/THESIS.200000730444

Degree

Doctor

Department

Department of New Biology

Publisher

DGIST

Show Full Item Record