Eun Seo Park. (2024). Unveiling fat depot-specific responses upon metabolic stimuli through single-cell and spatial transcriptome analysis. doi: 10.22677/THESIS.200000730444
Ⅰ. Introduction 1.1 Adipose Tissue · 1 1.2 Adipose tissue upon metabolic stimuli · 1 1.3 Adipose tissue at single cell resolution · 2 1.4 Aims and objectives · 3 IⅠ. Materials and methods 2.1 Animals 4 2.2 Human Subjects 4 2.3 scRNA-seq Library Preparation 4 2.4 scRNA-seq Data Procession · 5 2.5 scRNA-seq Data Analysis 6 2.6 Adipose Tissue Fractionation 7 2.7 Fluorescence-Activated Cell Sorting (FACS) 7 2.8 Isolation of SVFs from Human Adipose Tissue 7 2.9 Immunocytochemistry and Immunohistochemistry · 8 2.10 Isolation of Adipocyte Nuclei · 8 2.11 Cell Culture 9 2.12 Proliferation Assay · 9 2.13 Monocyte Migration Assay · 10 2.14 siRNA Treatment · 10 2.15 RT-qPCR · 11 2.16 Transplantation and Lymph Node (LN) Dissection · 11 2.17 Statistical Analysis 11 2.18 Spatial-seq Data Analysis 12 ⅠII. Results 3.1 Under homeostatic conditions, ASC clusters in IAT and EAT display distinct molecular properties and consist of three adipogenic stages 17 3.2 The adipogenic potential of ES1 and IS1 ASCs is primarily determined by their intrinsic factors 27 3.3 In EAT, obesogenic stimuli enhance ES1 ASC proliferation through FGF and TGFβ signaling, leading to de novo adipogenesis · 33 3.4 In obesity, EAT-specific SDC1+ regulate fibrosis and inflammation in a depot- specific manner 39 3.5 During obesity, IAT specific CXCL14+ ASCs exert a suppressive effect on monocyte infiltration 45 3.6 BST2high ASCs specific to IAT serve as precursors for beige adipocytes, and their biogenesis is under the regulation of lymph nodes (LNs) 53 ⅠV. Discussion Discussion · 65 Acknowledgement · 68 References · 69 요약문 79
