Unveiling fat depot-specific responses upon metabolic stimuli through single-cell and spatial transcriptome analysis
- Title
- Unveiling fat depot-specific responses upon metabolic stimuli through single-cell and spatial transcriptome analysis
- Author(s)
- Eun Seo Park
- DGIST Authors
- Eun Seo Park ; June M. Kwak ; Jong Kyoung Kim
- Advisor
- 곽준명
- Co-Advisor(s)
- Jong Kyoung Kim
- Issued Date
- 2024
- Awarded Date
- 2024-02-01
- Type
- Thesis
- Description
- single-cell RNA-seq;spatial transcriptome;white adipose tissue;brown adipose tissue;adipose stem cell
- Table Of Contents
-
Ⅰ. Introduction
1.1 Adipose Tissue · 1
1.2 Adipose tissue upon metabolic stimuli · 1
1.3 Adipose tissue at single cell resolution · 2
1.4 Aims and objectives · 3
IⅠ. Materials and methods
2.1 Animals 4
2.2 Human Subjects 4
2.3 scRNA-seq Library Preparation 4
2.4 scRNA-seq Data Procession · 5
2.5 scRNA-seq Data Analysis 6
2.6 Adipose Tissue Fractionation 7
2.7 Fluorescence-Activated Cell Sorting (FACS) 7
2.8 Isolation of SVFs from Human Adipose Tissue 7
2.9 Immunocytochemistry and Immunohistochemistry · 8
2.10 Isolation of Adipocyte Nuclei · 8
2.11 Cell Culture 9
2.12 Proliferation Assay · 9
2.13 Monocyte Migration Assay · 10
2.14 siRNA Treatment · 10
2.15 RT-qPCR · 11
2.16 Transplantation and Lymph Node (LN) Dissection · 11
2.17 Statistical Analysis 11
2.18 Spatial-seq Data Analysis 12
ⅠII. Results
3.1 Under homeostatic conditions, ASC clusters in IAT and EAT display distinct
molecular properties and consist of three adipogenic stages 17
3.2 The adipogenic potential of ES1 and IS1 ASCs is primarily determined by their
intrinsic factors 27
3.3 In EAT, obesogenic stimuli enhance ES1 ASC proliferation through FGF and TGFβ
signaling, leading to de novo adipogenesis · 33
3.4 In obesity, EAT-specific SDC1+ regulate fibrosis and inflammation in a depot-
specific manner 39
3.5 During obesity, IAT specific CXCL14+ ASCs exert a suppressive effect on
monocyte infiltration 45
3.6 BST2high ASCs specific to IAT serve as precursors for beige adipocytes, and their
biogenesis is under the regulation of lymph nodes (LNs) 53
ⅠV. Discussion
Discussion · 65
Acknowledgement · 68
References · 69
요약문 79
- URI
-
http://hdl.handle.net/20.500.11750/48004
http://dgist.dcollection.net/common/orgView/200000730444
- Degree
- Doctor
- Department
- Department of New Biology
- Publisher
- DGIST
- Related Researcher
-
-
Kwak, June Myoung
- Research Interests Calcium Signaling; ABA;ROS Signaling; plant development; Development of Abscission; Culluar Features; Signaling network; Environmental Regulation
-
- Files in This Item:
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- Appears in Collections:
- Department of New Biology Theses Ph.D.
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