A Study on the Structural & Functional Traits and Effects of Skin-Associated Proteins: Keratin 17 Tail and Erythropoietin
- Title
- A Study on the Structural & Functional Traits and Effects of Skin-Associated Proteins: Keratin 17 Tail and Erythropoietin
- Alternative Title
- 피부 연관 단백질(Keratin 17 Tail과 Erythropoietin)의 구조 기능적 특성 및 영향 연구
- Author(s)
- Suji Kim
- DGIST Authors
- Suji Kim ; Chang-Hun Lee ; Young-Sam Lee
- Advisor
- 이창훈
- Co-Advisor(s)
- Young-Sam Lee
- Issued Date
- 2024
- Awarded Date
- 2024-02-01
- Type
- Thesis
- Description
- skin; 피부;wound healing; 상처 회복;structure; 구조;keratin; 케라틴;erythropoietin; 에리트로포이에틴
- Table Of Contents
-
I. Introduction 1
II. Materials and methods 6
1. In vitro 6
1.1 Plasmids 6
1.2 Protein expression 6
1.3 Inclusion Body (IB) prep 7
1.4 Purification of proteins 7
1.5 Reconstitution of Keratin filament 8
1.6 Assessing keratin filament assemblies and binding 8
1.7 Electron microscope of keratin filaments and measurements of filament width 9
2. In vivo 9
2.1 Cell culture 9
2.2 Sample preparation for skin cell proliferation test 10
2.3 Hematoxylin and eosin (H&E) staining 10
2.4 Western blot 10
2.5 Measurement of the melanin amount 11
3. In slico 12
3.1 Complex structure modeling and analysis 12
4. Statistical analysis 12
III. Results 13
1. The K17 tail domain is essential for filament assembly in the K6/K17 pair 13
1.1 Protein purification for experiment 13
1.2 Sedimentation assay of reconstituted keratin pairs 15
1.3 TEM observations of reconstituted keratin pairs 18
1.4 Self-dimerization of K17T affects keratin filament assembly 19
2. EPO-derived peptides affect skin cell proliferation and melanin synthesis 22
2.1 EPOR is expressed in cells that compose the skin 22
2.2 Designing EPO-derived peptides to reduce side effects and modeling their binding structures to EPOR 23
2.3 Effect of EPO-derived peptides on skin cell proliferation and migration 26
2.4 Effects of EPO-derived peptides on melanin synthesis 31
IV. Discussion 34
1. The K17 tail domain is essential for filament assembly in the K6/K17 pair 34
2. EPO-derived peptides affect skin cell proliferation and melanin synthesis 36
V. Conclusion 37
VI. References 38
- URI
-
http://hdl.handle.net/20.500.11750/48061
http://dgist.dcollection.net/common/orgView/200000726587
- Degree
- Master
- Department
- Department of New Biology
- Publisher
- DGIST
- Related Researcher
-
-
Lee, Chang-Hun
- Research Interests Structure-Function relationship of cytoskeletal proteins and membrane proteins; Structure-based design of biomolecules and drugs; Development of drug delivery system in skin
-
- Files in This Item:
-
There are no files associated with this item.
- Appears in Collections:
- Department of New Biology Theses Master
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.