Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, A-Ram | - |
dc.contributor.author | Park, Tae Jung | - |
dc.contributor.author | Kim, Minseok S. | - |
dc.contributor.author | Kim, In-Ho | - |
dc.contributor.author | Kim, Ki-Suk | - |
dc.contributor.author | Chung, Kwang Hoe | - |
dc.contributor.author | Ko, Sungho | - |
dc.date.accessioned | 2018-01-25T01:05:50Z | - |
dc.date.available | 2018-01-25T01:05:50Z | - |
dc.date.created | 2017-08-09 | - |
dc.date.issued | 2017-05-15 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/5005 | - |
dc.description.abstract | A highly sensitive electrochemical immunosensor was developed by preventing electrode fouling and using a novel fusion protein of silica binding polypeptides (SBP)-protein G (ProG) created by recombinant DNA technology as a functional crosslinker for rapid and self-oriented immobilization of antibodies onto silica nanoparticles (SiNPs). Antibody immobilization onto the SiNPs by the SBP-ProG could rapidly be achieved without any chemical treatment. The immunosensor was fabricated through bonding of a partially gold-deposited cyclic olefin copolymer (COC) (top substrate) and gold patterned interdigitated array COC electrode (bottom substrate). To prevent electrode fouling, human immunoglobulin G (hIgG) was immobilized onto the ceiling inside the microchannel, instead of the bottom electrode. Alkaline phosphatase (AP)-labeled anti-hIgG was allowed to immunoreact with hIgG on the ceiling, followed by addition of an enzyme to generate an oxidative peak current. A three-fold increase in current was observed from the immunosensor without any electrode fouling compared with a control with the protein functionalized electrode. Also, the SiNPs facilely coated with AP-anti-hIgG via the SBP-ProG could increase the electrochemical signal up to 20% larger than that of the AP-anti-hIgG alone. Furthermore, this immunosensor was ultrasensitive with a detection limit of 0.68pg/mL of a biomarker associated with prostate cancer. © 2017 Elsevier B.V. | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Functional fusion proteins and prevention of electrode fouling for a sensitive electrochemical immunosensor | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.aca.2017.02.026 | - |
dc.identifier.scopusid | 2-s2.0-85014672806 | - |
dc.identifier.bibliographicCitation | Analytica Chimica Acta, v.967, pp.70 - 77 | - |
dc.subject.keywordAuthor | Electrochemical immunosensor | - |
dc.subject.keywordAuthor | Electrode fouling | - |
dc.subject.keywordAuthor | Silica binding polypeptide | - |
dc.subject.keywordAuthor | Protein G | - |
dc.subject.keywordAuthor | Silica nanoparticle | - |
dc.subject.keywordAuthor | Cyclic olefin copolymer | - |
dc.subject.keywordPlus | Alkalinity | - |
dc.subject.keywordPlus | Antibodies | - |
dc.subject.keywordPlus | Bins | - |
dc.subject.keywordPlus | Biosensors | - |
dc.subject.keywordPlus | Carbon Nanotubes | - |
dc.subject.keywordPlus | Chemical Bonds | - |
dc.subject.keywordPlus | Conformational Changes | - |
dc.subject.keywordPlus | Cyclic Olefin Copolymer | - |
dc.subject.keywordPlus | Cyclic Olefin Copolymers | - |
dc.subject.keywordPlus | Dendrimer | - |
dc.subject.keywordPlus | Diseases | - |
dc.subject.keywordPlus | Electro Chemical Electrodes | - |
dc.subject.keywordPlus | Electrochemical Immunosensor | - |
dc.subject.keywordPlus | Electrochemical Immunosensors | - |
dc.subject.keywordPlus | Electrode Fouling | - |
dc.subject.keywordPlus | Electrodes | - |
dc.subject.keywordPlus | Fouling | - |
dc.subject.keywordPlus | Gold | - |
dc.subject.keywordPlus | Gold Deposits | - |
dc.subject.keywordPlus | Gold Nanoparticles | - |
dc.subject.keywordPlus | Horseradish Peroxidase | - |
dc.subject.keywordPlus | Immunosensors | - |
dc.subject.keywordPlus | Nanoparticles | - |
dc.subject.keywordPlus | Olefins | - |
dc.subject.keywordPlus | Orientation | - |
dc.subject.keywordPlus | Phosphatases | - |
dc.subject.keywordPlus | Polypeptides | - |
dc.subject.keywordPlus | Prostate Specific Antigen | - |
dc.subject.keywordPlus | Protein G | - |
dc.subject.keywordPlus | Proteins | - |
dc.subject.keywordPlus | Recombinant Proteins | - |
dc.subject.keywordPlus | Silica | - |
dc.subject.keywordPlus | Silica Binding Polypeptide | - |
dc.subject.keywordPlus | Silica Nanoparticle | - |
dc.subject.keywordPlus | Silica Nanoparticles | - |
dc.subject.keywordPlus | Surface Concentration | - |
dc.citation.endPage | 77 | - |
dc.citation.startPage | 70 | - |
dc.citation.title | Analytica Chimica Acta | - |
dc.citation.volume | 967 | - |
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