Bacteriophages as Templates for Manufacturing Supramolecular Structures
- Title
- Bacteriophages as Templates for Manufacturing Supramolecular Structures
- Author(s)
- Korkmaz, Nuriye ; Kim, Young Jun ; Nam, Chang Hoon
- DGIST Authors
- Nam, Chang Hoon
- Issued Date
- 2013-03
- Type
- Article
- Article Type
- Article
- Subject
- Amino Acids ; Animal Cell ; Apatite ; Apatite-Like Materials ; Atomic Force Microscopy ; Bacteriophage ; Bacteriophages ; Bio-Compatible Materials ; Bioengineering ; Biomineralization ; Calcium ; Calcium Chloride ; Capsid Proteins ; Chemical Reduction ; Coat Proteins ; Cobalt ; Cobalt Chloride ; Controlled Study ; EDX Spectroscopy ; Electron Microscopy ; Energy Dispersive X Ray Spectroscopy ; Escherichia Coli ; Immunofluorescence Analysis ; Immunofluorescence Microscopy ; Macromolecular Substances ; Metallation ; Metallization ; Metallizing ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Molecular Scaffold ; N-Terminals ; Nanofabrication ; Negative Surface Charges ; Non-Human ; Phage Bundles ; Phage Display ; Phosphorus ; Recombinant Proteins ; Reduction ; Scanning Electron Microscopy ; Static Electricity ; Supramolecular Chemistry ; Supramolecular Structure ; Surface Properties ; Virus Assembly ; X Ray Spectrometry ; Zeta Potential ; Zeta Potential Measurements
- ISSN
- 1616-5187
- Abstract
- SS phages are genetically enginnered by replacing 2 N-terminal amino acids of the p8 coat protein of the fd phage. AGE and zeta potential measurements show that SS phages carry at least 1/4 less net negative surface charge than fd phages. Morphology and thickness of phages are studied at different counterion concentrations (10-3, 10-2 and 10-1M) by AFM, SEM and immunofluorescence analyses. Bundles induced by CoCl2 and CaCl2 are either metallized by chemical reduction or biomineralized for apatite-like material formation. EDX spectroscopy confirms the presence of Co, P and Ca peaks in mineralized samples. Such bottom-up manufactured phage scaffolds might be applied in bioengineering studies. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Publisher
- Wiley-VCH Verlag
- Related Researcher
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Nam, Chang-Hoon 뉴바이올로지학과
- Research Interests Protein Engineering; History and Philosophy of Science; Scientific Art; Biomaterial Development (Tissue Engineering) with modified bacteriophage; Bio Sensor Development
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- Appears in Collections:
- School of Undergraduate Studies 1. Journal Articles
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