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Bacteriophages as Templates for Manufacturing Supramolecular Structures

Title
Bacteriophages as Templates for Manufacturing Supramolecular Structures
Authors
Korkmaz, NuriyeKim, Young JunNam, Chang Hoon
DGIST Authors
Nam, Chang Hoon
Issue Date
2013-03
Citation
Macromolecular Bioscience, 13(3), 376-387
Type
Article
Article Type
Article
Keywords
Amino AcidsAnimal CellApatiteApatite-Like MaterialsAtomic Force MicroscopyBacteriophageBacteriophagesBio-Compatible MaterialsBioengineeringBiomineralizationCalciumCalcium ChlorideCapsid ProteinsChemical ReductionCoat ProteinsCobaltCobalt ChlorideControlled StudyEDX SpectroscopyElectron MicroscopyEnergy Dispersive X Ray SpectroscopyEscherichia ColiImmunofluorescence AnalysisImmunofluorescence MicroscopyMacromolecular SubstancesMetallationMetallizationMetallizingMicroscopy, Atomic ForceMicroscopy, Electron, ScanningMolecular ScaffoldN-TerminalsNanofabricationNegative Surface ChargesNon-HumanPhage BundlesPhage DisplayPhosphorusRecombinant ProteinsReductionScanning Electron MicroscopyStatic ElectricitySupramolecular ChemistrySupramolecular StructureSurface PropertiesVirus AssemblyX Ray SpectrometryZeta PotentialZeta Potential Measurements
ISSN
1616-5187
Abstract
SS phages are genetically enginnered by replacing 2 N-terminal amino acids of the p8 coat protein of the fd phage. AGE and zeta potential measurements show that SS phages carry at least 1/4 less net negative surface charge than fd phages. Morphology and thickness of phages are studied at different counterion concentrations (10-3, 10-2 and 10-1M) by AFM, SEM and immunofluorescence analyses. Bundles induced by CoCl2 and CaCl2 are either metallized by chemical reduction or biomineralized for apatite-like material formation. EDX spectroscopy confirms the presence of Co, P and Ca peaks in mineralized samples. Such bottom-up manufactured phage scaffolds might be applied in bioengineering studies. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
URI
http://hdl.handle.net/20.500.11750/5322
DOI
10.1002/mabi.201200290
Publisher
Wiley-VCH Verlag
Related Researcher
  • Author Nam, Chang Hoon  
  • Research Interests Protein Engineering; History and Philosophy of Science; Scientific Art; Biomaterial Development (Tissue Engineering) with modified bacteriophage; Bio Sensor Development
Files:
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Collection:
School of Undergraduate Studies1. Journal Articles


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