Bioinspired Technique for Antibody Immobilization on Various Surfaces

Abstract

Biosensors are vital for disease diagnosis and therapeutic monitoring, but many struggle with effectively immobilizing biological probes due to unsuitable chemical interactions. To solve this, we present a one-pot coating method inspired by marine organisms' adhesive mechanisms, utilizing the exceptional properties of polydopamine. Known for its hydrophilicity and versatile affinity for various substrates, polydopamine acts as a robust adhesive polymer, enhancing the attachment of neutravidin to sensor surfaces. This improves antibody immobilization through strong avidin-biotin interactions, leading to a significant increase in detection sensitivity. Validation with model biomarkers showed more than a tenfold improvement in sensitivity compared to commercial immuno-plates. This approach highlights the transformative potential of polydopamine in biosensor technology, offering a straightforward and effective solution to enhance biosensor performance in diverse biological applications.