WEB OF SCIENCE
SCOPUS
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 임평옥 | - |
| dc.contributor.author | Myeong Hoon Kang | - |
| dc.date.accessioned | 2025-02-28T21:01:00Z | - |
| dc.date.available | 2025-02-28T21:01:00Z | - |
| dc.date.issued | 2025 | - |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/57967 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000848182 | - |
| dc.description | Leaf senescence | - |
| dc.description.tableofcontents | List of Contents Abstract i List of Contents ii List of Tables v List of Figures vi I. Background introduction 1 1.1 Developmental transition during leaf lifespan 1 1.2 Chloroplast degeneration 3 1.3 Nucleus-Chloroplast communication in response to internal and external signal 6 1.4 Regulatory RNAs in plant biology 9 1.5 Multidimensional regulatory role of long noncoding RNA 11 1.6 Research goal and Specific aims 12 II. Chloroplast-Related LncRNA (CHLORELLA) mediates chloroplast functional transition across leaf aging. 13 2.1 Background 13 2.2 Results 13 2.2.1 Co-expression analysis of differentially expressed mRNAs and LncRNAs during leaf lifespan. 13 2.2.2 Chloroplast Related lncRNA (CHLORELLA) is canonical lncRNA. 19 2.2.3 CHLORELLA influences the transition of leaves to senescence 21 2.2.4 The transcriptome analysis in Col-0 and chlorella-1 reveals that CHLORELLA affects chloroplast related gene expression 27 2.2.5 CHLORELLA interacts with chloroplast related proteins. 31 2.2.6 CHLORELLA modulates PEP accumulation and its activity. 35 2.2.7 CHLORELLA translocases from nucleus to chloroplast through cytosol 44 2.2.8 CHLORELLA interacts with PEP component FLN1 in chloroplast. 51 2.3 Discussion 55 III. Transcriptional and post-transcriptional regulation of CHLORELLA-From GLK1/2 to miRNA863-3p during leaf lifespan 59 3.1 Background 59 3.2 Results 59 3.2.1 Identification of transcriptional regulator of CHLORELLA 59 3.2.2 GLK1 and GLK2 directly regulate CHLORELLA transcription 64 3.2.3 Transiently overexpressed CHLORELLA in glk1 glk2 protoplast rescue the PEP accumulation and PEP-dependent genes’ expression. 67 3.2.4 GLK1/2-CHLORELLA module regulates PEP accumulation and its activity along the aging 69 3.2.5 miRNA863-3p positively regulates leaf senescence. 74 3.2.6 miRNA863-3p suppresses CHLORELLA abundance by direct binding. 78 3.2.7 Genetic analysis of CHLORELLA-miRNA863-3p module in leaf senescence 83 3.3 Discussion 85 IV. Appendix 87 4.1 Hidden role of CHLORELLA: age-related resistance 87 4.1.1 Background 87 4.1.2 Results 88 4.1.3 Discussion 99 V. Materials & Methods in this thesis 101 5.1 Plant materials, preparation of constructs and transgenic plants, and growth conditions . 101 5.2 Analysis of leaf senescence 102 5.3 RNA extraction and gene expression analysis 102 5.4 Coding potential analysis 103 5.5 Transcriptome analysis in chlorella-1 104 5.6 Protein extraction and western blot analysis 104 5.7 RNA Pull-down assay 105 5.8 In-gel peptide preparation 107 5.9 LC-MS/MS 108 5.10 Mass spectrometry Database searching 108 5.11 Gene expression analysis in the chloroplast-enriched fraction 109 5.12 RNA immunoprecipitation (RIP) assay 110 5.13 Arabidopsis mesophyll protoplast isolation and transient expression analysis 112 5.14 Dual-luciferase assay 112 5.15 PEP assembly detection by Blue-Native electrophoresis (BN-PAGE) 113 5.16 Chromatin immunoprecipitation (ChIP)–seq analysis and ChIP-qPCR 114 5.17 Fluorescence microscopy using phage MS2 system 116 5.18 Fluorescence resonance energy transfer imaging 117 5.19 2D BN-PAGE/SDS-PAGE and identification of thylakoid proteins 118 5.20 Crosslinked RNA pull-down assay 120 5.21 5’ RNA Ligase Mediated Rapid Amplification of cDNA End ( 5’-RLM RACE) assay.. 121 5.22 Drawing schematic diagram 122 V.I References 126 |
- |
| dc.format.extent | 141 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | Molecular genetic study on the long noncoding RNA CHLORELLA-mediated regulatory network in leaf senescence | - |
| dc.title.alternative | long noncoding RNA CHLORELLA에 의한 노화조절 네트워크의 분자유전학적인 연구 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/THESIS.200000848182 | - |
| dc.description.degree | Doctor | - |
| dc.contributor.department | Department of New Biology | - |
| dc.identifier.bibliographicCitation | Myeong Hoon Kang. (2025). Molecular genetic study on the long noncoding RNA CHLORELLA-mediated regulatory network in leaf senescence. doi: 10.22677/THESIS.200000848182 | - |
| dc.contributor.coadvisor | Jong-Chan Lee | - |
| dc.date.awarded | 2025-02-01 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.ND 강34 202502 | - |
| dc.date.accepted | 2025-01-20 | - |
| dc.contributor.alternativeDepartment | 뉴바이올로지학과 | - |
| dc.subject.keyword | Leaf senescence | - |
| dc.contributor.affiliatedAuthor | Myeong Hoon Kang | - |
| dc.contributor.affiliatedAuthor | Pyung Ok Lim | - |
| dc.contributor.affiliatedAuthor | Jong-Chan Lee | - |
| dc.contributor.alternativeName | 강명훈 | - |
| dc.contributor.alternativeName | Pyung Ok Lim | - |
| dc.contributor.alternativeName | 이종찬 | - |
| dc.rights.embargoReleaseDate | 2027-02-28 | - |
