Yumin Kang. (2025). Microscale Magneto-kinetic systems for bio-linked particle control and cell screening. doi: 10.22677/THESIS.200000845134
Type
Thesis
Description
Magnetophoresis, Cell screening, Magnetic particle, Droplet microfluidics, Magnetic thin film
Table Of Contents
Ⅰ. Introduction 1 1.1 Motivation 1 1.2 Overview of the study 13 1.3 Theoretical background 15 1.3.1 Magnetophoretic-based manipulation 15 1.3.1.1 Fundamental principle of magnetophoresis 16 1.3.1.2 Micro-magnetophoretic circuits 18 1.3.2 Magnetically actuated droplet manipulation 25 1.3.2.1 Fundamental principle of magnetic droplet manipulation 30 1.3.2.2 Applications based on magnetic droplet manipulation 33 1.3.3 Cell screening method 35 1.3.3.1 Cell cytotoxicity assay 35 1.3.3.2 Doxorubicin mediated cell death 37 1.3.3.3 Calcium signaling 38 Ⅱ. Material and method 43 2.1 Fabrication of microscale magneto-kinetic system 43 2.1.1 Sputtering-based process 43 2.1.1.1 Photolithography 43 2.1.1.2 Direct-current magnetron sputtering 44 2.1.1.3 Lift-off and fabrication of passivation layer 44 2.1.2 Electrochemical deposition-based process 45 2.1.2.1 Fabrication of micro-pattern templet by photolithography 45 2.1.2.2 Electrochemical deposition 45 2.2 Integration of microfluidic and magneto-kinetic system 47 2.2.1 Fabrication of microfluidic channel 47 2.2.2 Droplet generating microfluidic system 48 2.3 Preparation of biochemical components 50 2.3.1 Antibody conjugation on micro magnetic particle for labeling cells 50 2.3.2 Conjugation of polystyrene beads to magnetic particles 51 2.3.3 Doxorubicin loading on nano magnetic particle for drug delivery 52 2.3.4 Drug response test using DOX-loaded nanoparticles (NPs) 53 2.4 Analysis equipment 54 2.4.1 Magneto-Optical Kerr Effect (MOKE) 54 2.4.2 Vibration Sample Magnetometer (VSM) 54 2.4.3 UV-vis spectrophotometer 55 2.4.4 Scanning Electron Microscope (SEM) 55 2.4.5 Atomic Force Microscope (AFM) 56 2.5 Analysis using simulations 56 2.5.1 Micromagnet simulation, Mumax3 56 2.6 Cell preparation 58 2.6.1 Cell culture and treatment 58 2.6.2 Binding cells to antibody-coated magnetic particles 58 2.6.3 Cell staining for viability assay 58 2.6.4 Live cell calcium imaging 59 2.7 Experimental setup 59 Ⅲ. Results and discussion 61 3.1 Selective cell sorting with parameters control 61 3.1.1 Size-based cell sorting by ellipse-shaped micromagnet 61 3.1.2 Trapping desired number of particles using topographic structure ·· 70 3.1.3 Conclusion 76 3.2 On-chip drug response test using nanoparticle 77 3.2.1 A systemic design for on-chip drug response test 77 3.2.2 Collection and transportation of MNPs 78 3.2.3 Drug loading and release characteristics of MNPs 80 3.2.4 Drug response test for potential Cell-on-chip applications 83 3.2.5 Conclusion 85 3.3 Droplet manipulation based on magneto-kinetic system 86 3.3.1 Design of a hybrid system for droplet manipulation 87 3.3.2 Adjusting magnetic force from magnetic disk 92 3.3.3 Droplet generation by microfluidic channel 98 3.3.4 Investigation of operating conditions for droplet manipulation 100 3.3.5 Conclusion 104 3.4 Cell-cell communication study 105 3.4.1 Calcium dynamics of MCF-7 in confined droplets 105 3.4.2 Calcium dynamics of HUVEC with stimulus in open space 107 3.4.3 Conclusion 109 Ⅳ. Conclusion 110 4.1 Conclusion of the works 110 References 113 Summary (in Korean) 124 List of publications 125
