WEB OF SCIENCE
SCOPUS
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 정찬 | - |
| dc.contributor.author | Jiwon Hwang | - |
| dc.date.accessioned | 2025-02-28T21:01:41Z | - |
| dc.date.available | 2025-02-28T21:01:41Z | - |
| dc.date.issued | 2025 | - |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/58016 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000844106 | - |
| dc.description | EZHIP, Metformin, H3K27me3, KDM6, Cancer Epigenetics | - |
| dc.description.tableofcontents | Abstract List of Contents Abbreviations List of Tables List of Figures Ⅰ. Introduction 1. EZHIP overexpression inhibits PRC2 in various cancers 2. PFA Ependymoma is a fatal pediatric brain tumor 3. Metformin treatment reverses EZHIP expression and restores H3K27me3 levels 4. KDM6 is the potential target of metformin treatment ⅠⅠ. Results 1. Effect of metformin on EZHIP-expressing cells 1.1. EZHIP levels are increased in U2OS 1.2. Metformin is cytotoxic to EZHIP-expressing cell lines 1.3. Metformin induces EZHIP reduction and H3K27me3 upregulation in EZHIP-expressing cells 1.4. Metformin alters EZHIP expression at the transcriptional level 2. Regulation of EZHIP by DNA Methylation 2.1. DNA demethylation induces EZHIP expression 2.2. Endogenous EZHIP expression is determined by DNA methylation 2.3. Metformin leads to subtle methylation in the EZHIP gene body 3. Regulation of EZHIP via KDM6 Family Reduction by Metformin 3.1. Metformin interacts with the histone demethylase KDM6 family 3.2. Metformin downregulates KDM6 family 3.3. KDM6A and KDM6B are potential targets for EZHIP reduction ⅠⅠⅠ. Discussion ⅠV. Conclusion and Future Perspective V. Method and Materials 1. Cell Culture 2. Cell Viability Assay 3. Histone Extraction 4. Western Blotting 5. Quantitative RT-PCR (RT-qPCR) 6. Bisulfite sequencing 7. Chromatin Immunoprecipitation RT-qPCR (ChIP-qPCR) 8. Transformation and Plasmid Preparation 9. Stable Cell Line Generation 10. siRNA Transfection 11. Quantification and Statistical Analysis References Summary (Korean) |
- |
| dc.format.extent | 50 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | EZH Inhibitory Protein (EZHIP) Regulation by Targeting Genetic and Epigenetic Alterations | - |
| dc.title.alternative | 유전학 및 후성유전학 변화를 통한 이즈힙 단백질의 조절 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/THESIS.200000844106 | - |
| dc.description.degree | Master | - |
| dc.contributor.department | Department of New Biology | - |
| dc.identifier.bibliographicCitation | Jiwon Hwang. (2025). EZH Inhibitory Protein (EZHIP) Regulation by Targeting Genetic and Epigenetic Alterations. doi: 10.22677/THESIS.200000844106 | - |
| dc.contributor.coadvisor | Young-Sam Lee | - |
| dc.date.awarded | 2025-02-01 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.NM 황78 202502 | - |
| dc.date.accepted | 2025-01-20 | - |
| dc.contributor.alternativeDepartment | 뉴바이올로지학과 | - |
| dc.subject.keyword | EZHIP, Metformin, H3K27me3, KDM6, Cancer Epigenetics | - |
| dc.contributor.affiliatedAuthor | Jiwon Hwang | - |
| dc.contributor.affiliatedAuthor | Chan Chung | - |
| dc.contributor.affiliatedAuthor | Young-Sam Lee | - |
| dc.contributor.alternativeName | 황지원 | - |
| dc.contributor.alternativeName | Chan Chung | - |
| dc.contributor.alternativeName | 이영삼 | - |
| dc.rights.embargoReleaseDate | 2027-02-28 | - |
