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  Department of New Biology Theses Master

Enhancing immunoproteasome-mediated MHC-I antigen presentation by targeting OAS1 in senescent cells

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dc.contributor.advisor 이병훈 -
dc.contributor.author Jeonghan Lee -
dc.date.accessioned 2026-01-23T10:54:49Z -
dc.date.available 2026-01-23T10:54:49Z -
dc.date.issued 2026 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/59652 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200000942489 -
dc.description Immunoproteasome, cellular senescence, immune surveillance, IRF1, OAS1 -
dc.description.tableofcontents Ⅰ. Introduction 1
1.1 Cellular senescence 1
1.2 Immune surveillance of senescent cells 2
1.3 Immunoproteasome 5
1.4 Interferon gamma signaling in the MHC-I antigen presentation process 8
1.5 Oligoadenylate synthetase 10
1.6 Hypothesis and specific aims 13

II. Results 14
2.1 Immunoproteasome expression and activity are less induced upon IFNγ treatment in various senescent models 14
2.2 Low IRF1 protein level is attributed to reduced immunoproteasome induction in senescent cells 16
2.3 IFNγ-induced IRF1 translation is downregulated in senescent cells 18
2.4 OAS1-mediated IRF1 translation may be hindered by dsRNA and OAS3 in senescent cells 20
2.5 Surface HLA-I expression is less induced after IFNγ treatment in senescent cells 23

III. Discussion 25

IV. Materials and Methods 27
4.1 Cell culture 27
4.2 Induction of cellular senescence 27
4.3 Senescence-associated β-galactosidase staining 27
4.4 Western blot analysis 27
4.5 Proteasome activity measurement from whole cell lysates 28
4.6 RNA extraction and gene expression analysis 29
4.7 mRNA decay measurement 29
4.8 RNA immunoprecipitation 30
4.9 Immunocytochemistry 31
4.10 RNA FISH staining 31
4.11 Flow cytometry 32
4.12 Statistical analysis 33

V. References 36		 -
dc.format.extent 41 -
dc.language eng -
dc.publisher DGIST -
dc.title Enhancing immunoproteasome-mediated MHC-I antigen presentation by targeting OAS1 in senescent cells -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200000942489 -
dc.description.degree Master -
dc.contributor.department Department of New Biology -
dc.contributor.coadvisor Il-Kyu Choi -
dc.date.awarded 2026-02-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.NM 이74 202602 -
dc.date.accepted 2026-01-19 -
dc.contributor.alternativeDepartment 뉴바이올로지학과 -
dc.subject.keyword Immunoproteasome, cellular senescence, immune surveillance, IRF1, OAS1 -
dc.contributor.affiliatedAuthor Jeonghan Lee -
dc.contributor.affiliatedAuthor Byung-Hoon Lee -
dc.contributor.affiliatedAuthor Il-Kyu Choi -
dc.contributor.alternativeName 이정한 -
dc.contributor.alternativeName Byung-Hoon Lee -
dc.contributor.alternativeName 최일규 -
dc.rights.embargoReleaseDate 2031-02-28 -
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