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  Department of New Biology Theses Master

Characterization of the Interaction between Human Protamine 1 and DNA

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dc.contributor.advisor 김유리 -
dc.contributor.author Seongmin Kim -
dc.date.accessioned 2026-09-01T19:31:22Z -
dc.date.available 2026-09-01T19:31:22Z -
dc.date.issued 2026 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/60774 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200001018031 -
dc.description protamine, sperm, spermiogenesis, DNA interaction -
dc.description.tableofcontents I. Introduction 1
II. Materials and Methods 6
2.1 hP1 expression plasmid 6
2.2 Protein expression and purification of hP1 6
2.3 Western blot analysis 7
2.4 Preparation of DNA 8
2.5 Electrophoretic mobility shift assay （EMSA） 8
2.6 Preparation of single-molecule DNA substrates 9
2.7 Single-molecule imaging assay 9
2.8 Analysis of kymograph 11
III. Results 13
3.1 Expression and purification of hP1 13
3.2 Effect of DNA GC content on hP1 binding affinity 18
3.3 Estimation of DNA length bound by a single hP1 molecule 20
3.4 DNA condensation depending on hP1 concentration 22
IV. Discussion 26
References 30
Summary in Korean 35		 -
dc.format.extent 35 -
dc.language eng -
dc.publisher DGIST -
dc.title Characterization of the Interaction between Human Protamine 1 and DNA -
dc.title.alternative 인간 프로타민 1과 디옥시리보핵산 상호작용 특성 분석 -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200001018031 -
dc.description.degree Master -
dc.contributor.department Department of New Biology -
dc.contributor.coadvisor Chan Chung -
dc.date.awarded 2026-08-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.NM 김54 202608 -
dc.date.accepted 2026-07-21 -
dc.contributor.alternativeDepartment 뉴바이올로지학과 -
dc.subject.keyword protamine, sperm, spermiogenesis, DNA interaction -
dc.contributor.affiliatedAuthor Seongmin Kim -
dc.contributor.affiliatedAuthor Yoori Kim -
dc.contributor.affiliatedAuthor Chan Chung -
dc.contributor.alternativeName 김성민 -
dc.contributor.alternativeName Yoori Kim -
dc.contributor.alternativeName 정찬 -
dc.rights.embargoReleaseDate 2031-08-31 -
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