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  Department of New Biology Theses Master

Characterization of cohesin dynamics mediated by STAG variants and SMC3 acetylation

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dc.contributor.advisor 김유리 -
dc.contributor.author Minseok Seo -
dc.date.accessioned 2026-09-01T19:31:27Z -
dc.date.available 2026-09-01T19:31:27Z -
dc.date.issued 2026 -
dc.identifier.uri https://scholar.dgist.ac.kr/handle/20.500.11750/60776 -
dc.identifier.uri http://dgist.dcollection.net/common/orgView/200001018065 -
dc.description cohesin, STAG1/2, SMC3 acetylation, insect cell-based purification, cohesin dynamics -
dc.description.tableofcontents Ⅰ. Introduction 1
II. Materials and Methods 11
2.1 Expression plasmids for cohesin subunits 11
2.2 Site-directed mutagenesis for SMC3 K105Q / K106Q （SMC3 QQ mutant） 11
2.3 Generation of STAG2 expression plasmid 12
2.4 Blue-white screening 13
2.5 Isolation of recombinant Bacmid DNA and analyze by PCR 14
2.6 Baculovirus production and amplification in Sf9 insect cells 15
2.7 Protein expression in High-Five insect cells 16
2.8 Cohesin variants complex purification 16
2.9 Electrophoretic mobility shift assay （EMSA） 18
2.10 ATPase assay 19
Ⅲ. Results 21
3.1 Cohesin complex expression using Bac-to-Bac baculovirus system 21
3.2 Purification of cohesin variant complexes 27
3.3 SMC3 acetylation-mimic state reduces cohesin ATPase activity 32
3.4 STAG variants and SMC3 acetylation regulate cohesin DNA binding 34
3.5 STAG1 and STAG2 directly bind DNA with distinct binding behavior 36
3.6 DNA binding preferences of STAG1 and STAG2 39
Ⅳ. Discussion 41
References 47
Summary in Korean 57		 -
dc.format.extent 57 -
dc.language eng -
dc.publisher DGIST -
dc.title Characterization of cohesin dynamics mediated by STAG variants and SMC3 acetylation -
dc.title.alternative STAG 변이체와 SMC3 아세틸화에 의한 코헤신 동역학의 특성 비교 -
dc.type Thesis -
dc.identifier.doi 10.22677/THESIS.200001018065 -
dc.description.degree Master -
dc.contributor.department Department of New Biology -
dc.contributor.coadvisor Jin Hae Kim -
dc.date.awarded 2026-08-01 -
dc.publisher.location Daegu -
dc.description.database dCollection -
dc.citation XT.NM 서38 202608 -
dc.date.accepted 2026-07-21 -
dc.contributor.alternativeDepartment 뉴바이올로지학과 -
dc.subject.keyword cohesin, STAG1/2, SMC3 acetylation, insect cell-based purification, cohesin dynamics -
dc.contributor.affiliatedAuthor Minseok Seo -
dc.contributor.affiliatedAuthor Yoori Kim -
dc.contributor.affiliatedAuthor Jin Hae Kim -
dc.contributor.alternativeName 서민석 -
dc.contributor.alternativeName Yoori Kim -
dc.contributor.alternativeName 김진해 -
dc.rights.embargoReleaseDate 2031-08-31 -
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