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Single quantum dot tracking reveals the impact of nanoparticle surface on intracellular state

Title
Single quantum dot tracking reveals the impact of nanoparticle surface on intracellular state
Author(s)
Zahid, Mohammad U.Ma, LiangLim, Sung JunSmith, Andrew M.
DGIST Authors
Lim, Sung Jun
Issued Date
2018-05
Type
Article
Article Type
Article
Subject
Cell deliveryNanobiotechnologyNanoparticlesQuantum dotsSingle-molecule biophysics
ISSN
2041-1723
Abstract
Inefficient delivery of macromolecules and nanoparticles to intracellular targets is a major bottleneck in drug delivery, genetic engineering, and molecular imaging. Here we apply live-cell single-quantum-dot imaging and tracking to analyze and classify nanoparticle states after intracellular delivery. By merging trajectory diffusion parameters with brightness measurements, multidimensional analysis reveals distinct and heterogeneous populations that are indistinguishable using single parameters alone. We derive new quantitative metrics of particle loading, cluster distribution, and vesicular release in single cells, and evaluate intracellular nanoparticles with diverse surfaces following osmotic delivery. Surface properties have a major impact on cell uptake, but little impact on the absolute cytoplasmic numbers. A key outcome is that stable zwitterionic surfaces yield uniform cytosolic behavior, ideal for imaging agents. We anticipate that this combination of quantum dots and single-particle tracking can be widely applied to design and optimize next-generation imaging probes, nanoparticle therapeutics, and biologics. © 2018 The Author(s).
URI
http://hdl.handle.net/20.500.11750/6393
DOI
10.1038/s41467-018-04185-w
Publisher
Nature Publishing Group
Related Researcher
Files in This Item:
10.1038_s41467-018-04185-w.pdf

10.1038_s41467-018-04185-w.pdf

기타 데이터 / 1.65 MB / Adobe PDF download
Appears in Collections:
Intelligent Devices and Systems Research Group 1. Journal Articles

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