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Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells

Title
Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells
Authors
Seo, Eun JinPark, Jae KyungJeong, HoimKang, Jung SookKim, Hyung RyongJang, Il Ho
DGIST Authors
Kim, Hyung Ryong
Issue Date
2018-06
Citation
International Journal of Oral Biology, 43(2), 77-82
Type
Article
Article Type
Article
Keywords
dental pulp stem cellorganoid cultureendothelial cellCD31
ISSN
1226-7155
Abstract
The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated from the molars (DPCs), in order to establish the organoid culture. DPCs were established after growing pulp cells in an MSC expansion media (MSC-EM). DPCs were subjected to organoid growth media (OGM) in comparison with human dental pulp stem cells (DPSCs). Inside the extracellular matrix in the OGM, the DPCs and DPSCs readily formed vessel-like structures, which were not observed in the MSC-EM. Immunocytochemistry analysis and flow cytometry analysis showed the elevated expression of CD31 in the DPCs and DPSCs cultured in the OGM. These results suggest endothelial cell-prone differentiation of the DPCs and DPSCs in organoid culture condition.
URI
http://hdl.handle.net/20.500.11750/6669
Publisher
대한구강생물학회
Files:
There are no files associated with this item.
Collection:
ETC1. Journal Articles


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