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Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform
- Electrochemical biosensor for Mycobacterium tuberculosis DNA detection based on gold nanotubes array electrode platform
- Torati, Ramulu; Reddy, Venu; Yoon, Seok Soo; Kim, CheolGi
- Issue Date
- Aromatic compounds; Biosensors; Cyclic voltammetry; DNA; Electrodeposition; Electrodes; Gold; Nanotubes; Reduction; Scanning electron microscopy; Synthesis (chemical); Thick films; Yarn; DNA biosensors; Electrochemical biosensor; Electrochemical deposition; Electrochemical DNA biosensors; Electrochemical performance; Hybridization; Methylene Blue; Mycobacterium tuberculosis; Electrochemical electrodes; bacterial DNA; complementary DNA; gold nanotube; nanotube; unclassified drug; Article; biosensor; cyclic potentiometry; DNA determination; DNA hybridization; DNA probe; electrochemical analysis; electron transport; gold nanotube array; limit of detection; nanoarray; nanofabrication; nonhuman
- The template assisted electrochemical deposition technique was used for the synthesis of gold nanotubes array (AuNTsA). The morphological structure of the synthesized AuNTsA was observed by scanning electron microscopy and found that the individual nanotubes are around 1.5 mu m in length with a diameter of 200 nm. Nanotubes are vertically aligned to the Au thick film, which is formed during the synthesis process of nanotubes. The electrochemical performance of the AuNTsA was compared with the bare Au electrode and found that AuNTsA has better electron transfer surface than bare Au electrode which is due to the high surface area. Hence, the AuNTsA was used as an electrode for the fabrication of DNA hybridization biosensor for detection of Mycobacterium Tuberculosis DNA. The DNA hybridization biosensor constructed by AuNTsA electrode was characterized by cyclic voltammetry technique with Fe (CN)(6)(3-/4-) as an electrochemical redox indicator. The selectivity of the fabricated biosensor was illustrated by hybridization with complementary DNA and non-complementary DNA with probe DNA immobilized AuNTsA electrode using methylene blue as a hybridization indicator. The developed electrochemical DNA biosensor shows good linear range of complementary DNA concentration from 0.01 ng/mu L to 100 ng/mu L with high detection limit. (C) 2015 Elsevier B.V. All rights reserved.
- Elsevier Ltd
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