Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Byeong Yeon | ko |
dc.contributor.author | Oh, Se In | ko |
dc.contributor.author | Shin, Su Yeon | ko |
dc.contributor.author | Yim, Sang Gu | ko |
dc.contributor.author | Yang, Seung Yun | ko |
dc.contributor.author | Hahn, Young Ki | ko |
dc.contributor.author | Choi, Sung Young | ko |
dc.date.accessioned | 2018-08-06T07:08:42Z | - |
dc.date.available | 2018-08-06T07:08:42Z | - |
dc.date.created | 2018-08-06 | - |
dc.date.issued | 2018-07 | - |
dc.identifier.citation | Analytical Chemistry, v.90, no.13, pp.8254 - 8260 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/9046 | - |
dc.description.abstract | Major challenges of miniaturizing flow cytometry include obviating the need for bulky, expensive, and complex pump-based fluidic and laser-based optical systems while retaining the ability to detect target cells based on their unique surface receptors. We addressed these critical challenges by (i) using a viscous liquid additive to control flow rate passively, without external pumping equipment, and (ii) adopting an immunobead assay that can be quantified with a portable fluorescence cell counter based on a blue light-emitting diode. Such novel features enable pumpless microflow cytometry (pFC) analysis by simply dropping a sample solution onto the inlet reservoir of a disposable cell-counting chamber. With our pFC platform, we achieved reliable cell counting over a dynamic range of 9-298 cells/μL. We demonstrated the practical utility of the platform by identifying a type of cancer cell based on CD326, the epithelial cell adhesion molecule. This portable microflow cytometry platform can be applied generally to a range of cell types using immunobeads labeled with specific antibodies, thus making it valuable for cell-based and point-of-care diagnostics. Copyright © 2018 American Chemical Society. | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.subject | CIRCULATING TUMOR-CELLS | - |
dc.subject | FLOW-CYTOMETRY | - |
dc.subject | STEM-CELLS | - |
dc.subject | SHEATHLESS | - |
dc.subject | SORTER | - |
dc.title | Pumpless Microflow Cytometry Enabled by Viscosity Modulation and Immunobead Labeling | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.8b01804 | - |
dc.identifier.wosid | 000438008600064 | - |
dc.identifier.scopusid | 2-s2.0-85048362515 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.contributor.nonIdAuthor | Kim, Byeong Yeon | - |
dc.contributor.nonIdAuthor | Oh, Se In | - |
dc.contributor.nonIdAuthor | Shin, Su Yeon | - |
dc.contributor.nonIdAuthor | Yim, Sang Gu | - |
dc.contributor.nonIdAuthor | Yang, Seung Yun | - |
dc.contributor.nonIdAuthor | Choi, Sung Young | - |
dc.identifier.citationVolume | 90 | - |
dc.identifier.citationNumber | 13 | - |
dc.identifier.citationStartPage | 8254 | - |
dc.identifier.citationEndPage | 8260 | - |
dc.identifier.citationTitle | Analytical Chemistry | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | N | - |
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