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Promotion of formyl peptide receptor 1-mediated neutrophil chemotactic migration by antimicrobial peptides isolated from the centipede Scolopendra subspinipes mutilans

Title
Promotion of formyl peptide receptor 1-mediated neutrophil chemotactic migration by antimicrobial peptides isolated from the centipede Scolopendra subspinipes mutilans
Authors
Park, YJ[Park, Yoo Jung]Lee, SK[Lee, Sung Kyun]Jung, YS[Jung, Young Su]Lee, MY[Lee, Mingyu]Lee, HY[Lee, Ha Young]Kim, SD[Kim, Sang Doo]Park, JS[Park, Joon Seong]Koo, JH[Koo, JaeHyung]Hwang, JS[Hwang, Jae Sam]Bae, YS[Bae, Yoe-Sik]
DGIST Authors
Koo, JH[Koo, JaeHyung]
Issue Date
2016-09-30
Citation
BMB Reports, 49(9), 520-525
Type
Article
Article Type
Article
Keywords
Anti-Microbial PeptideChemotaxisFormyl Peptide Receptor 1NeutrophilScolopendra Subspinipes Mutilans
ISSN
1976-6696
Abstract
We investigated the effects of two antimicrobial peptides (AMPs) isolated from Scolopendra subspinipes mutilans on neutrophil activity. Stimulation of mouse neutrophils with the two AMPs elicited chemotactic migration of the cells in a pertussis toxin-sensitive manner. The two AMPs also stimulated activation of ERK and Akt, which contribute to chemotactic migration of neutrophils. We found that AMP-stimulated neutrophil chemotaxis was blocked by a formyl peptide receptor (FPR) 1 antagonist (cyclosporin H); moreover the two AMPs stimulated the chemotactic migration of FPR1-expressing RBL-2H3 cells but not of vector-expressing RBL- 2H3 cells. We also found that the two AMPs stimulate neutrophil migration in vivo, and that this effect is blocked in FPR1-deficient mice. Taken together, our results suggest that the two AMPs stimulate neutrophils, leading to chemotactic migration through FPR1, and the two AMPs will be useful for the study of FPR1 signaling and neutrophil activation. © 2016 by the The Korean Society for Biochemistry and Molecular Biology.
URI
http://hdl.handle.net/20.500.11750/2193
DOI
10.5483/BMBRep.2016.49.9.098
Publisher
Korean Society for Molecular and Cellular Biology
Related Researcher
  • Author Koo, Jae Hyung The Koo Lab - ChemoReception Laboratory(CRLab)
  • Research Interests
Files:
There are no files associated with this item.
Collection:
New BiologyETC1. Journal Articles


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