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Refining a Pathogen's Genome to Know the Enemy Better for the Winning Battles

Title
Refining a Pathogen's Genome to Know the Enemy Better for the Winning Battles
Authors
Mohammad, Hazara BegumLee, Seung-EunKim, Min-Sik
DGIST Authors
Kim, Min-Sik
Issue Date
2019-05
Citation
Proteomics, 19(9), 1900005
Type
Article
Article Type
Editorial Material; Note
Author Keywords
genome annotationnovel gene discoverypathogenicityproteogenomics
Keywords
gene amplificationinfectious agentmass spectrometrynonhumannotepathogenicitypolymerase chain reactionproteogenomicsreverse transcription
ISSN
1615-9853
Abstract
Clavibacter michiganensis, subsp. michiganens is a gram positive bacterial pathogen infecting tomato, resulting in the great losses of yield and quality worldwide. Despite its great impact on economics, we do not fully understand the virulence factors from its genome probably due to imperfect genome annotation. Peritore-Galve et al. utilized proteogenomic approach to identify 26 novel protein-coding regions, to extend 59 annotated open reading frames and to confirm protein expression for ≈70% of predicted gene models of Clavibacter michiganensis subsp. michiganensis. New findings by the proteogenomics technique were further confirmed using reverse transcription-polymerase chain reaction. To win battles with pathogens, it is advantageous to know about our enemies accurately which can be achieved by mass spectrometry-based identifications of novel protein-coding regions in genomes. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
URI
http://hdl.handle.net/20.500.11750/10083
DOI
10.1002/pmic.201900005
Publisher
John Wiley & Sons Ltd.
Related Researcher
  • Author Kim, Min-Sik Laboratory for QBIO and Precision Medicine
  • Research Interests Cancer Proteogenomics, Biomarker Discovery, Integrative Multi-Omics
Files:
There are no files associated with this item.
Collection:
Department of New BiologyLaboratory for QBIO and Precision Medicine1. Journal Articles


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