Cited 4 time in webofscience Cited 3 time in scopus

Accurate Background Subtraction in STED Nanoscopy by Polarization Switching

Title
Accurate Background Subtraction in STED Nanoscopy by Polarization Switching
Authors
Lee, Jong-ChanMa, YeHan, Kyu YoungHa, Taekjip
DGIST Authors
Lee, Jong-Chan
Issue Date
2019-07
Citation
ACS Photonics, 6(7), 1789-1797
Type
Article
Article Type
Article
Author Keywords
super-resolution microscopySTED nanoscopybackground noisebackground suppressionoptical resolution
Keywords
MICROSCOPY
ISSN
2330-4022
Abstract
STED nanoscopy helped usher in resolution revolution of optical microscopy and has been widely used to study subcellular structures and dynamics. To achieve optical resolution beyond the diffraction limit, STED nanoscopy employs 10(4)-10(5) times more intense light compared to conventional imaging methods, giving rise to additional background that can be detrimental to imaging and quantification of dense samples. Here, we report a simple and easy-to-implement method to suppress background noise by polarization switching (psSTED). STED imaging requires a well-defined polarization state of a STED laser (typically left-circular polarization). By switching to right-circular polarization, the spatial profile of the STED laser changes to fill up the center of the donut-shaped beam, enabling an accurate background recording. Through a straightforward subtraction of the recorded background from the regular STED image, we achieved background-subtracted STED imaging. With simulation and experiment, we demonstrate that psSTED outperforms other background subtraction methods.
URI
http://hdl.handle.net/20.500.11750/10402
DOI
10.1021/acsphotonics.9b00537
Publisher
American Chemical Society
Related Researcher
  • Author Lee, Jong-Chan Single-molecule Biophysics and Advanced Bioimaging Laboratory
  • Research Interests Liquid-liquid phase separation; Super-resolution imaging;
Files:
There are no files associated with this item.
Collection:
Department of New BiologySingle-molecule Biophysics and Advanced Bioimaging Laboratory1. Journal Articles


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