Title

Study on pheromone-mediated developmental processes in C. elegans

DGIST Authors

Kim, Kyuhyung ; Park, Jisoo ; Lee, Seung-Jae

Advisor

김규형

Co-Advisor(s)

Seung-Jae Lee

Issued Date

2019

Awarded Date

2019-02

Citation

Jisoo Park. (2019). Study on pheromone-mediated developmental processes in C. elegans. doi: 10.22677/thesis.200000171525

Type

Thesis

Table Of Contents

List of Contents
Abstract ··································································· i
List of contents ······················································ ⅲ
List of figures ························································· ⅴ
Ⅰ. INTRODUCTION
1.1 Pheromone-mediated gene expression plasticity in C. elegans
············································································· 1
1.2 Pheromone-mediated developmental plasticity ·············· 2
Ⅱ. MATERIALS AND METHODS ······························· 5
Ⅲ. RESULTS
3.1 Neuropeptide signaling regulates pheromone-me diated gene
expression of a chemoreceptor gene in C. elegans
3.1.1 Pheromone-mediated str-3 repression in the ASI chemosensory
neurons was imposed from egg to the L1 larval stage ·· 16
3.1.2 Mutations in the pheromone receptor genes abolished pheromone-
mediated regulation of str-3 gene expression ··········· 18
3.1.3 Food availability, internal feeding state, and ambient temperature
did not affect str-3 expression ····························· 25
3.1.4 FLP-18 neuropeptide signaling was required for pheromonemediated
regulation of str-3 gene expression via a NPR-1 neuropeptide
receptor ························································· 27
3.1.5 Pheromone exposure did not exhibit abnormal chemotactic behavior
································································ 32
3.2 Neuropeptide signaling regulates pheromone-mediated gene expression
of a chemoreceptor gene in C. elegans
3.2.1 crh-1 CREB mutants inappropriately form transient pre-daue
L2d larvae ··························································· 34
3.2.2 L2d formation of crh-1 mutants is specifically induced by
ascr#5 exposure even in plenty food supply ···················· 37
3.2.3 crh-1CREB mutants express a L2d marker gene in non-dauer ivinducing
condition ················································· 39
3.2.4 crh-1 acts in the ASI neurons to regulate ascr#5-mediated L2d
formation ···························································· 44
3.2.5 The DAF-7 TGF-β signaling pathway regulates ascr#5-medi
ated L2d formation of crh-1 mutants ···························· 50
3.2.6 daf-7 expression is decreased in the ASI neurons of crh-1
mutants ······························································ 55
3.2.7 CRH-1 directly regulates daf-7 expression via a conserved
CRE motif ··························································· 60
3.2.8 crh-1 may interact with several genes to mediate proper L2d
formation ···························································· 64
Ⅳ. DISCUSSION ···················································· 66
Ⅴ. REFERENCES ··················································· 78
Ⅵ. SUMMARY IN KOREANS ··································· 81

URI

http://dgist.dcollection.net/common/orgView/200000171525
http://hdl.handle.net/20.500.11750/10675

DOI

10.22677/thesis.200000171525

Degree

DOCTOR

Department

Brain and Cognitive Sciences

Publisher

DGIST

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