The physiological role of insulin synthesized in paraventricular nucleus of the hypothalamus
- Title
- The physiological role of insulin synthesized in paraventricular nucleus of the hypothalamus
- Author(s)
- Jaemeun Lee
- DGIST Authors
- Kim, Eun-Kyoung ; Lee, Jaemeun ; Yoo, Joo-Yeon
- Advisor
- 김은경
- Co-Advisor(s)
- Joo-Yeon Yoo
- Issued Date
- 2019
- Awarded Date
- 2019-02
- Type
- Thesis
- Table Of Contents
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I. Introduction 1
1. Insulin in the brain 1
2. Hypothalamus 2
3. Wnt/β-catenin signaling in the hypothalamus 2
4. Stress and hypothalamus 3
II. Materials and Methods 6
1. Animals 6
2. Cell culture and treatments 7
3. Quantitative real-time polymerase chain reaction (qRT-PCR) 7
4. Enzyme-linked immunosorbent assay (ELISA) 7
5. Immunofluorescence analysis 8
6. Immunoblot analysis 9
7. Lentivirus preparation 9
8. Stereotaxic injection of Wnt3a and lentivirus 10
9. in situ hybridization 11
10. Microdissection of hypothalamic sub regions 11
11. Statistical analysis 11
III. Results 14
1. Induction mechanism of insulin in the hypothalamus 14
1.1. Insulin is expressed in the brain 14
1.2. Metabolic challenges do not change insulin expression in the hypothalamus 14
1.3. Wnt3a increases the production of insulin in the hypothalamic cells, N39 16
1.4. Wnt3a upregulates insulin production through the canonical Wnt/β-catenin signaling 19
1.5. Wnt3a increases insulin production through NeuroD1 21
1.6. Wnt3a administration increases the expression of Ins2 and NeuroD1 in the hypothalamus 28
2. The physiological role of insulin synthesized in paraventricular nucleus of the hypothalamus (PVN) 30
2.1. Insulin is synthesized in the PVN 30
2.2. PVN insulin is needed to maintain pituitary GH production 30
2.3. PVN insulin regulates body length of young mice via modulating GH production 34
2.4. Acute restraint stress (RS) reduces GH production via suppression of PVN insulin 34
2.5. Chronic RS-induced suppression of PVN insulin results in growth retardation of young mice via reduction of GH production 39
IV. Discussion 44
IIV. Reference 50
- URI
-
http://dgist.dcollection.net/common/orgView/200000171520
http://hdl.handle.net/20.500.11750/10679
- Degree
- DOCTOR
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
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Kim, Eun-Kyoung
- Research Interests Neural functions in metabolic diseases; 뇌신경세포와 비만; 당뇨 등의 대사 질환 관련 연구
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- Appears in Collections:
- Department of Brain Sciences Theses Ph.D.
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