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Characterization of Synaptoporin in the Hippocampal Mossy Fiber Pathway

Title
Characterization of Synaptoporin in the Hippocampal Mossy Fiber Pathway
Authors
Kim, Sang Hee
DGIST Authors
Kim, Sang Hee; Lee, Kea Joo; Ko, Jaewon
Advisor(s)
고재원
Co-Advisor(s)
Lee, Kea Joo
Issue Date
2019
Available Date
2019-10-03
Degree Date
2019-02
Type
Thesis
Table Of Contents
Ⅰ. Introduction 1 1.1 Synaptoporin expression in the mossy fiber pathway 1 1.2 Synaptic vesicle pools 2 1.3 Potential roles of synaptoporin in the mossy fiber pathway 3 Ⅱ. Material & Methods 5 2.1 Experimental animals 5 2.2 Primary hippocampal neuron culture 5 2.3 Immunocytochemistry (ICC) 6 2.4 SRRF (Super resolution radial fluctuations) imaging 6 2.5 Immunogold Electron Microscopy 7 2.6 TEM (Transmission electron microscopy) imaging 8 2.7 Viral vector / Stereotaxic virus injection 8 2.8 Immunohistochemistry (IHC) 9 2.9 CLSM (Confocal laser scanning microscopy) imaging 9 2.10 Image processing / Statistics 10 Ⅲ. Results 11 3.1 Synaptoporinlocalization at the synapse level (pre vs. post synaptic region) 11 3.2 Ultrastructural distribution of synaptoporin in the mossy fiber terminals 12 3.3 Synaptic remodeling by modulation of SPO levels 12 IⅤ. Discussion 19 4.1 Discussion 19
URI
http://dgist.dcollection.net/common/orgView/200000171528
http://hdl.handle.net/20.500.11750/10696
DOI
10.22677/thesis.200000171528
Degree
MASTER
Department
Brain and Cognitive Sciences
University
DGIST
Related Researcher
  • Author Ko, Jaewon Laboratory of Synapse Formation and Function
  • Research Interests Synapse Formation and Function; Neural Circuits; 뇌질환; animal model
Files:
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Collection:
Department of Brain and Cognitive SciencesThesesMaster


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