Title

Characterization of Synaptoporin in the Hippocampal Mossy Fiber Pathway

DGIST Authors

Ko, Jaewon ; Kim, Sang Hee ; Lee, Kea Joo

Advisor

고재원

Co-Advisor(s)

Lee, Kea Joo

Issued Date

2019

Awarded Date

2019-02

Citation

Kim, Sang Hee. (2019). Characterization of Synaptoporin in the Hippocampal Mossy Fiber Pathway. doi: 10.22677/thesis.200000171528

Type

Thesis

Table Of Contents

Ⅰ. Introduction 1
1.1 Synaptoporin expression in the mossy fiber pathway 1
1.2 Synaptic vesicle pools 2
1.3 Potential roles of synaptoporin in the mossy fiber pathway 3
Ⅱ. Material & Methods 5
2.1 Experimental animals 5
2.2 Primary hippocampal neuron culture 5
2.3 Immunocytochemistry (ICC) 6
2.4 SRRF (Super resolution radial fluctuations) imaging 6
2.5 Immunogold Electron Microscopy 7
2.6 TEM (Transmission electron microscopy) imaging 8
2.7 Viral vector / Stereotaxic virus injection 8
2.8 Immunohistochemistry (IHC) 9
2.9 CLSM (Confocal laser scanning microscopy) imaging 9
2.10 Image processing / Statistics 10
Ⅲ. Results 11
3.1 Synaptoporinlocalization at the synapse level (pre vs. post synaptic region) 11
3.2 Ultrastructural distribution of synaptoporin in the mossy fiber terminals 12
3.3 Synaptic remodeling by modulation of SPO levels 12
IⅤ. Discussion 19
4.1 Discussion 19

URI

http://dgist.dcollection.net/common/orgView/200000171528
http://hdl.handle.net/20.500.11750/10696

DOI

10.22677/thesis.200000171528

Degree

MASTER

Department

Brain and Cognitive Sciences

Publisher

DGIST

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