Title

Endocytosis of BDNF and its localization in the astrocyte

DGIST Authors

Choe, Han Kyoung ; Yoon, Sungryeong ; Park, Hyungju

Advisor

최한경

Co-Advisor(s)

Hyungju Park

Issued Date

2019

Awarded Date

2019-02

Citation

Sungryeong Yoon. (2019). Endocytosis of BDNF and its localization in the astrocyte. doi: 10.22677/thesis.200000171531

Type

Thesis

Table Of Contents

I. Introduction 1
II. Materials and methods 3
2.1 Primary cell culture and transfection 3
2.2 Viral vectors for TrkB knockdown 3
2.3 Immunocytochemistry 3
2.4 Preparation of QD-BDNF conjugate 4
2.5 Preparation of extracellular solution for live-cell imaging 4
2.6 Live cell imaging 4
2.7 Data analysis 4
III. Results 6
3.1 QD-mBDNF as a tool for monitoring endosomal mature BDNF in astrocytes 6
3.2 Optimal QD-mBDNF treatment condition for observing endosomal BDNF 6
3.3 Endosomal pathways processing QD-mBDNF in astrocytes 7
3.4 Transport of endocytosed QD-mBDNF is affected by increase in intracellular Ca2+ 8
IV. Discussion 9
V. Figure legends 11
VI. Figures 14
References 26
Abstract in Korea 28

URI

http://dgist.dcollection.net/common/orgView/200000171531
http://hdl.handle.net/20.500.11750/10700

DOI

10.22677/thesis.200000171531

Degree

MASTER

Department

Brain and Cognitive Sciences

Publisher

DGIST

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