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Endocytosis of BDNF and its localization in the astrocyte

Title
Endocytosis of BDNF and its localization in the astrocyte
Authors
Sungryeong Yoon
DGIST Authors
Yoon, Sungryeong; Park, Hyungju; Choe, Han Kyoung
Advisor(s)
최한경
Co-Advisor(s)
Hyungju Park
Issue Date
2019
Available Date
2019-10-03
Degree Date
2019-02
Type
Thesis
Table Of Contents
I. Introduction 1 II. Materials and methods 3 2.1 Primary cell culture and transfection 3 2.2 Viral vectors for TrkB knockdown 3 2.3 Immunocytochemistry 3 2.4 Preparation of QD-BDNF conjugate 4 2.5 Preparation of extracellular solution for live-cell imaging 4 2.6 Live cell imaging 4 2.7 Data analysis 4 III. Results 6 3.1 QD-mBDNF as a tool for monitoring endosomal mature BDNF in astrocytes 6 3.2 Optimal QD-mBDNF treatment condition for observing endosomal BDNF 6 3.3 Endosomal pathways processing QD-mBDNF in astrocytes 7 3.4 Transport of endocytosed QD-mBDNF is affected by increase in intracellular Ca2+ 8 IV. Discussion 9 V. Figure legends 11 VI. Figures 14 References 26 Abstract in Korea 28
URI
http://dgist.dcollection.net/common/orgView/200000171531
http://hdl.handle.net/20.500.11750/10700
DOI
10.22677/thesis.200000171531
Degree
MASTER
Department
Brain and Cognitive Sciences
University
DGIST
Related Researcher
  • Author Choe, Han Kyoung Laboratory of Animal Behavior and Circadian rhythm
  • Research Interests Modulation of neural circuit; Circadian regulation of behavior and perception; Neurotechnology
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Collection:
Department of Brain and Cognitive SciencesThesesMaster


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