Title

Presenilin-2 (PS2) mediates corticosterone-induced autophagic cell death in adult hippocampal neural stem cells

DGIST Authors

Yu, Seong-Woon ; Hong, Jihyun ; Jang, Jae Eun

Advisor

유성운

Co-Advisor(s)

Jae-Eun Jang

Issued Date

2019

Awarded Date

2019-02

Citation

Jihyun Hong. (2019). Presenilin-2 (PS2) mediates corticosterone-induced autophagic cell death in adult hippocampal neural stem cells. doi: 10.22677/thesis.200000171535

Type

Thesis

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Material and Methods 7
2.1 Reagents and antibodies 7
2.2 Cell culture 8
2.3 Construction of CRISPR/Cas9-mediated KO cells 8
2.4 Transfection, Expression vectors 9
2.5 Cell death assay 9
2.6 Western blotting 10
2.7 Quantitative real-time polymerase chain reaction (qRT-PCR) 11
2.8 Statistical analysis 12
Ⅲ. Results 13
3.1 CORT increases ACD, not apoptosis in HCN stem cells 13
3.2 CORT increases PS2 expression in HCN stem cells 15
3.3 Knockout of PS2 prevents CORT-induced ACD 17
3.4 The rescue of PS2 increases CORT-induced ACD 19
3.5 Autophagy initiation step is intact in PS2 KO 20
3.6 LC3 modification is impaired by PS2 KO 23
Ⅳ. Discussion 26
Ⅴ. Reference 30
Abstract in Korean 36

URI

http://dgist.dcollection.net/common/orgView/200000171535
http://hdl.handle.net/20.500.11750/10703

DOI

10.22677/thesis.200000171535

Degree

MASTER

Department

Brain and Cognitive Sciences

Publisher

DGIST

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