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Presenilin-2 (PS2) mediates corticosterone-induced autophagic cell death in adult hippocampal neural stem cells

Title
Presenilin-2 (PS2) mediates corticosterone-induced autophagic cell death in adult hippocampal neural stem cells
Authors
Jihyun Hong
DGIST Authors
Hong, Jihyun; Jang, Jae EunYu, Seong-Woon
Advisor(s)
유성운
Co-Advisor(s)
Jae-Eun Jang
Issue Date
2019
Available Date
2019-10-03
Degree Date
2019-02
Type
Thesis
Table Of Contents
Ⅰ. Introduction 1 Ⅱ. Material and Methods 7 2.1 Reagents and antibodies 7 2.2 Cell culture 8 2.3 Construction of CRISPR/Cas9-mediated KO cells 8 2.4 Transfection, Expression vectors 9 2.5 Cell death assay 9 2.6 Western blotting 10 2.7 Quantitative real-time polymerase chain reaction (qRT-PCR) 11 2.8 Statistical analysis 12 Ⅲ. Results 13 3.1 CORT increases ACD, not apoptosis in HCN stem cells 13 3.2 CORT increases PS2 expression in HCN stem cells 15 3.3 Knockout of PS2 prevents CORT-induced ACD 17 3.4 The rescue of PS2 increases CORT-induced ACD 19 3.5 Autophagy initiation step is intact in PS2 KO 20 3.6 LC3 modification is impaired by PS2 KO 23 Ⅳ. Discussion 26 Ⅴ. Reference 30 Abstract in Korean 36
URI
http://dgist.dcollection.net/common/orgView/200000171535
http://hdl.handle.net/20.500.11750/10703
DOI
10.22677/thesis.200000171535
Degree
MASTER
Department
Brain and Cognitive Sciences
University
DGIST
Related Researcher
  • Author Jang, Jae Eun Advanced Electronic Devices Research Group(AEDRG) - Jang Lab.
  • Research Interests Nanoelectroinc device; 생체 신호 센싱 시스템 및 생체 모방 디바이스; 나노 통신 디바이스
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Collection:
Department of Brain and Cognitive SciencesThesesMaster


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