Cited 5 time in webofscience Cited 6 time in scopus

Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR

Title
Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR
Authors
Piamsomboon, PatharapolChoi, Seong-KyoonHanggono, BambangNuraini, Yani LestariWati, FatmaTang, Kathy F. J.Park, SongKwak, DongmiRhee, Man HeeHan, Jee EunKim, Ji Hyung
DGIST Authors
Choi, Seong-Kyoon
Issue Date
2019-12
Citation
Pathogens, 8(4), 233
Type
Article
Article Type
Article
Author Keywords
Enterocytozoon hepatopenaeiqPCRmicrosporidianshrimp parasite
Keywords
IN-SITU HYBRIDIZATIONVANNAMEIPATHOGENSMONODON
ISSN
2076-0817
Abstract
We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
URI
http://hdl.handle.net/20.500.11750/10943
DOI
10.3390/pathogens8040233
Publisher
MDPI
Related Researcher
Files:
Collection:
Division of Biotechnology1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE