Cited 1 time in webofscience Cited 2 time in scopus

Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads

Title
Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads
Authors
Lee, Seung JoonSim, Tae SeokShin, Hyun YoungLee, Jeong MinKim, Min. Young.Sunoo, JosephLee, Jeong-GunYea, KyungmooKim, Young ZoonVan Noort, D.Park, Soo KyungKim, Woon-HaePark, Kyun WooKim, Minseok S.
DGIST Authors
Yea, Kyungmoo; Kim, Woon-Hae; Kim, Minseok S.
Issue Date
2019-10
Citation
PLoS ONE, 14(10), e0223193
Type
Article
Article Type
Article
Keywords
THERAPYSIZEPOPULATIONSBIOMARKERMETASTATIC BREAST-CANCERISOLATION PLATFORMEFFICIENT CAPTUREPERIPHERAL-BLOOD
ISSN
1932-6203
Abstract
Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 μm high and 27.9 mm wide slit to capture cancer cells bound to 3 μm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 μm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs. © 2019 Lee et al.
URI
http://hdl.handle.net/20.500.11750/10981
DOI
10.1371/journal.pone.0223193
Publisher
Public Library of Science
Related Researcher
Files:
Collection:
Department of New BiologyProtein Engineering Lab1. Journal Articles
Department of New BiologyETC1. Journal Articles
Department of New BiologyBioDr. Lab - Nanobiomedicine1. Journal Articles


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