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Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads
- Microslit on a chip: A simplified filter to capture circulating tumor cells enlarged with microbeads
- Lee, Seung Joon; Sim, Tae Seok; Shin, Hyun Young; Lee, Jeong Min; Kim, Min. Young.; Sunoo, Joseph; Lee, Jeong-Gun; Yea, Kyungmoo; Kim, Young Zoon; Van Noort, D.; Park, Soo Kyung; Kim, Woon-Hae; Park, Kyun Woo; Kim, Minseok S.
- DGIST Authors
- Yea, Kyungmoo; Kim, Woon-Hae; Kim, Minseok S.
- Issue Date
- PLoS ONE, 14(10), e0223193
- Article Type
- THERAPY; SIZE; POPULATIONS; BIOMARKER; METASTATIC BREAST-CANCER; ISOLATION PLATFORM; EFFICIENT CAPTURE; PERIPHERAL-BLOOD
- Microchips are widely used to separate circulating tumor cells (CTCs) from whole blood by virtues of sophisticated manipulation for microparticles. Here, we present a chip with an 8 μm high and 27.9 mm wide slit to capture cancer cells bound to 3 μm beads. Apart from a higher purity and recovery rate, the slit design allows for simplified fabrication, easy cell imaging, less clogging, lower chamber pressure and, therefore, higher throughput. The beads were conjugated with anti-epithelial cell adhesion molecules (anti-EpCAM) to selectively bind to breast cancer cells (MCF-7) used to spike the whole blood. The diameter of the cell-bead construct was in average 23.1 μm, making them separable from other cells in the blood. As a result, the cancer cells were separated from 5 mL of whole blood with a purity of 52.0% and a recovery rate of 91.1%, and also we confirmed that the device can be applicable to clinical samples of human breast cancer patients. The simple design with microslit, by eliminating any high-aspect ratio features, is expected to reduce possible defects on the chip and, therefore, more suitable for mass production without false separation outputs. © 2019 Lee et al.
- Public Library of Science
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