Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Sung, Gihyun | ko |
dc.contributor.author | Lee, Song-Yi | ko |
dc.contributor.author | Kang, Myeong-Gyun | ko |
dc.contributor.author | Kim, Kyung Lock | ko |
dc.contributor.author | An, Jaeyeon | ko |
dc.contributor.author | Sim, Jaehwan | ko |
dc.contributor.author | Kim, Sungwan | ko |
dc.contributor.author | Kim, Seung Jun | ko |
dc.contributor.author | Ko, Jaewon | ko |
dc.contributor.author | Rhee, Hyun-Woo | ko |
dc.contributor.author | Park, Kyeng Min | ko |
dc.contributor.author | Kim, Kimoon | ko |
dc.date.accessioned | 2020-03-12T12:26:55Z | - |
dc.date.available | 2020-03-12T12:26:55Z | - |
dc.date.created | 2020-01-29 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.citation | Chemical Communications, v.56, no.10, pp.1549 - 1552 | - |
dc.identifier.issn | 1359-7345 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/11518 | - |
dc.description.abstract | In accordance with the rapid increase in demand for selective and spatial chemical tagging, and accurate detection of proteins of interest, we develop a sensitive protein detection method, termed "Supra-blot" capitalizing on high-affinity host-guest interaction between cucurbit[7]uril (CB[7]) and adamantylammonium (AdA). The method can directly detect chemically tagged proteins without false-positive signals caused by endogenous biomolecules. Not only a single specific protein, but also spatially localized proteins in cells were labeled with AdA, and selectively detected by a host molecule-enzyme hybrid, CB[7]-conjugated horseradish peroxidase (CB[7]-HRP) generating amplified chemiluminescence signals. This study shows the great potential of Supra-blot for accurate and reliable detection of proteins of interest in cells. © 2020 The Royal Society of Chemistry. | - |
dc.language | English | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Supra-blot: an accurate and reliable assay for detecting target proteins with a synthetic host molecule-enzyme hybrid | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/C9CC09699J | - |
dc.identifier.wosid | 000511328200015 | - |
dc.identifier.scopusid | 2-s2.0-85079021947 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.contributor.nonIdAuthor | Sung, Gihyun | - |
dc.contributor.nonIdAuthor | Lee, Song-Yi | - |
dc.contributor.nonIdAuthor | Kang, Myeong-Gyun | - |
dc.contributor.nonIdAuthor | Kim, Kyung Lock | - |
dc.contributor.nonIdAuthor | An, Jaeyeon | - |
dc.contributor.nonIdAuthor | Sim, Jaehwan | - |
dc.contributor.nonIdAuthor | Kim, Sungwan | - |
dc.contributor.nonIdAuthor | Rhee, Hyun-Woo | - |
dc.contributor.nonIdAuthor | Park, Kyeng Min | - |
dc.contributor.nonIdAuthor | Kim, Kimoon | - |
dc.identifier.citationVolume | 56 | - |
dc.identifier.citationNumber | 10 | - |
dc.identifier.citationStartPage | 1549 | - |
dc.identifier.citationEndPage | 1552 | - |
dc.identifier.citationTitle | Chemical Communications | - |
dc.type.journalArticle | Article | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | ENDOGENOUS BIOTIN | - |
dc.subject.keywordPlus | STREPTAVIDIN | - |
dc.subject.keywordPlus | TAG | - |
dc.subject.keywordPlus | TECHNOLOGY | - |
dc.subject.keywordPlus | PEROXIDASE | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | AZIDES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PAIR | - |
dc.contributor.affiliatedAuthor | Ko, Jaewon | - |
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