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Establishment of research platform to study glial contribution to neuronal dendritic development

Title
Establishment of research platform to study glial contribution to neuronal dendritic development
Translated Title
신경 수지상 발달에 대한 신경교 기여를 연구하기 위한 연구 플랫폼 구축
Authors
Yoonha Kim
DGIST Authors
Kim, Yoonha; Kim, Ho Min; Lee, Sung Bae
Advisor(s)
이성배
Co-Advisor(s)
Ho Min Kim
Issue Date
2020
Available Date
2020-06-07
Degree Date
2020-02
Type
Thesis
Description
glia, neuronal dendrites, golgi
Abstract
Glia are increasingly regarded to play an active role in the nervous system and no longer viewed as mere providers of passive and supportive responses to neurons. Glia contribute to nervous system both directly, by wrapping the axon of neurons, and indirectly, by regulating surrounding environment. While the glial effect on axonal development or maintenance is well studied, less is known about glial contribution to neuronal dendrites. Herein, I showed that the development of stereotypical neuronal dendrites depends on the existence of healthy and morphologically stable glia. I established an applied research platform using existing methods. Because of the diffi-culty to study the effects of non-cell autonomous influences in other model systems, I made the best use of the advantages of Drosophila model system. An independent enhancer-driven cellular marker allows to observe the alterations of neurons when glia are specifically manipu-lated. Furthermore, caspase-LOV system enable to control the degeneration process spatio-temporally in Drosophila. Taken together, the results show the diverse possibilities of glia contribution to neuronal dendrites
Table Of Contents
List of Contents Abstract ·································································································· i List of contents ······················································································· ii List of figures ·························································································· vi Ⅰ. INTRODUCTION 1 1.1 Glia is indispensable for neuronal development. ····················································· 1 1.2 Manipulation of glial integrity via light-controlled glial degeneration in Drosophila model system ··························································································· 2 II. MATERIALS AND METHODS ....................................................................................4 III. RESULTS 8 3.1 Changes in neuronal dendrites upon glial degeneration ........................................ 8 3.2 Changes in neuronal dendrites via blocking the function of glial Golgi .................. 15 IV. DISCUSSION .......................................................................................................... 29 V. REFERENCES .......................................................................................................... 31 VI. SUMMARY (국문요약) ............................................................................................ 33
URI
http://dgist.dcollection.net/common/orgView/200000285087
http://hdl.handle.net/20.500.11750/11912
DOI
10.22677/Theses.200000285087
Degree
Master
Department
Brain and Cognitive Sciences
University
DGIST
Related Researcher
  • Author Lee, Sung Bae Laboratory of Neurodegenerative Diseases and Aging
  • Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
Files:
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Collection:
Department of Brain and Cognitive SciencesThesesMaster


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