Title

Identification of Novel Rodent Proprioceptive receptor Using Proprioceptor-Specific CRISPR/Cas9 Genome Editing

DGIST Authors

Choe, Han Kyoung ; Kim, Eujung ; Kim, Kyuhyung

Advisor

최한경

Co-Advisor(s)

Kyuhyung Kim

Issued Date

2020

Awarded Date

2020-02

Citation

Eujung Kim. (2020). Identification of Novel Rodent Proprioceptive receptor Using Proprioceptor-Specific CRISPR/Cas9 Genome Editing. doi: 10.22677/Theses.200000285830

Type

Thesis

Description

Proprioception, Proprioceptive receptor, CRISPR/Cas9,

Table Of Contents

I. Introduction 1
II. Materials and Methods 4
III. Results 8
3.1 Evolutionally conserved TRP channel family from C. elegans to Human. 8
3.2 Production of CRISPR/Cas9-mediated knockdown vector against ortholog genes of C. elegans proprioceptive channels. 8
3.3 Establishment of intrathecal AAV delivery to the DRG. 9
3.4 Characterization of proprioception-related behavioral phenotype. 10
3.5 In vivo AAV-gRNA delivery and behavior results. 11
IV. Discussion 23
V. References 26

URI

http://dgist.dcollection.net/common/orgView/200000285830
http://hdl.handle.net/20.500.11750/11913

DOI

10.22677/Theses.200000285830

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

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