Title

Investigating the role of astrocytic cholesterol transport in neuronal APP expression using human iPSCs.

Alternative Title

인간 유도 만능 줄기 세포를 이용한 아포E단백질 대립 유전자에 따른 성상세포 유래 콜레스테롤이 신경세포의 아밀로이드 전구체 단백질 발현에 미치는 영향 연구

DGIST Authors

Seo, Jinsoo ; Kim, Jaekwang ; Jeong, Woojin

Advisor

서진수

Co-Advisor(s)

Jaekwang Kim

Issued Date

2020

Awarded Date

2020-02

Citation

Woojin Jeong. (2020). Investigating the role of astrocytic cholesterol transport in neuronal APP expression using human iPSCs. doi: 10.22677/Theses.200000287183

Type

Thesis

Description

Alzheimer's disease (AD), amyloid precursor protein (APP), Aβ, apolipoprotein E (APOE), Lipid rafts, Cholesterol, human induced pluripotent stem cells (hiPSCs)

Table Of Contents

Ⅰ. Introduction 1
Ⅱ. Material and Methods 5
2.1 Reagents and antibodies 5
2.2 Cell culture 7
2.3 Production of recombinant lentiviruses 8
2.4 Differentiation 9
2.5 Co-culture system 12
2.6 Drug treatment 13
2.7 Immunostaining analysis 13
2.8 Filipin staining 14
2.9 Aβ ELISA 14
2.10 Statistical analysis 15

Ⅲ. Results 18
3.1 Differences in cholesterol release by APOE genotypes 18
3.2 Increase lipid rafts and APP in rat neuron by cholesterol 20
3.3 Regulation of lipid rafts in rat neuron by APOE genotype of hAstrocyte 25
3.4 Regulation of lipid rafts and APP in the hiPSC-derived neuron by cholesterol 29
3.5 Regulation of lipid rafts in hNeuron by hAstrocyte APOE genotype 32
3.5 Calculate the value of APP density in co-localization using formula 36

Ⅳ. Discussion 39
Ⅴ. Reference 42
Abstract in Korean 45

URI

http://dgist.dcollection.net/common/orgView/200000287183
http://hdl.handle.net/20.500.11750/11917

DOI

10.22677/Theses.200000287183

Degree

Master

Department

Brain and Cognitive Sciences

Publisher

DGIST

Show Full Item Record