Investigating the role of astrocytic cholesterol transport in neuronal APP expression using human iPSCs.
- Title
- Investigating the role of astrocytic cholesterol transport in neuronal APP expression using human iPSCs.
- Alternative Title
- 인간 유도 만능 줄기 세포를 이용한 아포E단백질 대립 유전자에 따른 성상세포 유래 콜레스테롤이 신경세포의 아밀로이드 전구체 단백질 발현에 미치는 영향 연구
- Author(s)
- Woojin Jeong
- DGIST Authors
- Seo, Jinsoo ; Kim, Jaekwang ; Jeong, Woojin
- Advisor
- 서진수
- Co-Advisor(s)
- Jaekwang Kim
- Issued Date
- 2020
- Awarded Date
- 2020-02
- Type
- Thesis
- Description
- Alzheimer's disease (AD), amyloid precursor protein (APP), Aβ, apolipoprotein E (APOE), Lipid rafts, Cholesterol, human induced pluripotent stem cells (hiPSCs)
- Table Of Contents
-
Ⅰ. Introduction 1
Ⅱ. Material and Methods 5
2.1 Reagents and antibodies 5
2.2 Cell culture 7
2.3 Production of recombinant lentiviruses 8
2.4 Differentiation 9
2.5 Co-culture system 12
2.6 Drug treatment 13
2.7 Immunostaining analysis 13
2.8 Filipin staining 14
2.9 Aβ ELISA 14
2.10 Statistical analysis 15
Ⅲ. Results 18
3.1 Differences in cholesterol release by APOE genotypes 18
3.2 Increase lipid rafts and APP in rat neuron by cholesterol 20
3.3 Regulation of lipid rafts in rat neuron by APOE genotype of hAstrocyte 25
3.4 Regulation of lipid rafts and APP in the hiPSC-derived neuron by cholesterol 29
3.5 Regulation of lipid rafts in hNeuron by hAstrocyte APOE genotype 32
3.5 Calculate the value of APP density in co-localization using formula 36
Ⅳ. Discussion 39
Ⅴ. Reference 42
Abstract in Korean 45
- URI
-
http://dgist.dcollection.net/common/orgView/200000287183
http://hdl.handle.net/20.500.11750/11917
- Degree
- Master
- Department
- Brain and Cognitive Sciences
- Publisher
- DGIST
- Related Researcher
-
-
Seo, Jinsoo
- Research Interests iPSC; Alzheimer's disease; Neurodegeneration; Synapse; Neuroscience
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- Files in This Item:
-
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- Appears in Collections:
- Department of Brain Sciences Theses Master
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