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Investigating the role of astrocytic cholesterol transport in neuronal APP expression using human iPSCs.

Title
Investigating the role of astrocytic cholesterol transport in neuronal APP expression using human iPSCs.
Translated Title
인간 유도 만능 줄기 세포를 이용한 아포E단백질 대립 유전자에 따른 성상세포 유래 콜레스테롤이 신경세포의 아밀로이드 전구체 단백질 발현에 미치는 영향 연구
Authors
Woojin Jeong
DGIST Authors
Jeong, Woojin; Kim, Jaekwang; Seo, Jinsoo
Advisor(s)
서진수
Co-Advisor(s)
Jaekwang Kim
Issue Date
2020
Available Date
2020-06-12
Degree Date
2020-02
Type
Thesis
Description
Alzheimer's disease (AD), amyloid precursor protein (APP), Aβ, apolipoprotein E (APOE), Lipid rafts, Cholesterol, human induced pluripotent stem cells (hiPSCs)
Table Of Contents
Ⅰ. Introduction 1 Ⅱ. Material and Methods 5 2.1 Reagents and antibodies 5 2.2 Cell culture 7 2.3 Production of recombinant lentiviruses 8 2.4 Differentiation 9 2.5 Co-culture system 12 2.6 Drug treatment 13 2.7 Immunostaining analysis 13 2.8 Filipin staining 14 2.9 Aβ ELISA 14 2.10 Statistical analysis 15 Ⅲ. Results 18 3.1 Differences in cholesterol release by APOE genotypes 18 3.2 Increase lipid rafts and APP in rat neuron by cholesterol 20 3.3 Regulation of lipid rafts in rat neuron by APOE genotype of hAstrocyte 25 3.4 Regulation of lipid rafts and APP in the hiPSC-derived neuron by cholesterol 29 3.5 Regulation of lipid rafts in hNeuron by hAstrocyte APOE genotype 32 3.5 Calculate the value of APP density in co-localization using formula 36 Ⅳ. Discussion 39 Ⅴ. Reference 42 Abstract in Korean 45
URI
http://dgist.dcollection.net/common/orgView/200000287183
http://hdl.handle.net/20.500.11750/11917
DOI
10.22677/Theses.200000287183
Degree
Master
Department
Brain and Cognitive Sciences
University
DGIST
Related Researcher
  • Author Seo, Jinsoo Laboratory of Aging Brain
  • Research Interests iPSC; Alzheimer's disease; Neurodegeneration; Synapse; Neuroscience
Files:
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Collection:
Department of Brain and Cognitive SciencesThesesMaster


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