Alzheimer's disease (AD), amyloid precursor protein (APP), Aβ, apolipoprotein E (APOE), Lipid rafts, Cholesterol, human induced pluripotent stem cells (hiPSCs)
Table Of Contents
Ⅰ. Introduction 1 Ⅱ. Material and Methods 5 2.1 Reagents and antibodies 5 2.2 Cell culture 7 2.3 Production of recombinant lentiviruses 8 2.4 Differentiation 9 2.5 Co-culture system 12 2.6 Drug treatment 13 2.7 Immunostaining analysis 13 2.8 Filipin staining 14 2.9 Aβ ELISA 14 2.10 Statistical analysis 15
Ⅲ. Results 18 3.1 Differences in cholesterol release by APOE genotypes 18 3.2 Increase lipid rafts and APP in rat neuron by cholesterol 20 3.3 Regulation of lipid rafts in rat neuron by APOE genotype of hAstrocyte 25 3.4 Regulation of lipid rafts and APP in the hiPSC-derived neuron by cholesterol 29 3.5 Regulation of lipid rafts in hNeuron by hAstrocyte APOE genotype 32 3.5 Calculate the value of APP density in co-localization using formula 36
Ⅳ. Discussion 39 Ⅴ. Reference 42 Abstract in Korean 45