Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 서병창 | - |
| dc.contributor.author | Park, Cheon-Gyu | - |
| dc.date.accessioned | 2020-06-14T16:00:18Z | - |
| dc.date.available | 2020-06-14T16:00:18Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.uri | http://dgist.dcollection.net/common/orgView/200000281554 | en_US |
| dc.identifier.uri | http://hdl.handle.net/20.500.11750/11920 | - |
| dc.description | Voltage-gated calcium (CaV) channel, CaV β subunit, phosphatidylinositol 4,5-bisphosphate (PIP2), molecular gating mechanism | - |
| dc.description.statementofresponsibility | prohibition | - |
| dc.description.tableofcontents | Abstract ……………………………………………………………………………………………………………………… i List of contents …………………………………………………………………………………………………………… iii List of tables ………………………………………………………………………………………………………………… v List of figures ………………………………………………………………………………………………………………… v 1. INTRODUCTION ………………………………………………………………………………………………………… 1 2. MATERIALS AND METHODS ………………………………………………………………………………………… 8 2-1. cDNA …………………………………………………………………………………………………………………… 8 2-2. Molecular cloning …………………………………………………………………………………………………… 8 2-3. Cell culture and transfection ……………………………………………………………………………………… 9 2-4. Electrophysiology …………………………………………………………………………………………………… 10 2-5. Confocal imaging …………………………………………………………………………………………………… 10 2-6. Preparation of liposomes ………………………………………………………………………………………… 11 2-7. Assay for peptide-liposome binding ……………………………………………………………………………… 11 2-8. Curve fitting ………………………………………………………………………………………………………… 11 2-9. Förster resonance energy transfer (FRET) ……………………………………………………………………… 12 2-10. Statistical analysis …………………………………………………………………………………………………… 12 3. RESULTS …………………………………………………………………………………………………………………… 15 3-1. The HOOK region of β2 subunit regulates inactivation kinetics and PIP2 sensitivity of CaV2.2 channels ………………………………………………………………………………………………………… 15 3-2. Charged amino acids in the HOOK region mainly determine gating properties of CaV2.2 channels ………………………………………………………………………………………………………… 27 3-3. The short form of the HOOK region in β2c-short subunit shows similar regulatory effects on PIP2 sensitivity and inactivation of CaV2.2 channels ……………………………………………………………… 35 3-4. The B-domain of the HOOK region tethers β subunit to plasma membrane via electrostatic and hydrophobic interactions …………………………………………………………………………………………… 38 3-5. Distance between plasma membrane and CaV β subunit is critically important for modulating inactivation kinetics and PIP2 sensitivity of CaV2.2 channels …………………………………………………… 46 3-6. Conformational shift of I-II loop mainly determines gating properties of CaV2.2 channels ………… 53 3-7. Polybasic residues at the C-terminal end of I-II loop mainly determine PIP2 and muscarinic modulation of CaV2.2 channels ………………………………………………………………………………………… 66 4. DISCUSSION……………………………………………………………………………………………………………… 72 5. REFERENCES …………………………………………………………………………………………………………… 84 6. SUMMARY IN KOREAN ……………………………………………………………………………………………… 92 |
- |
| dc.format.extent | 104 | - |
| dc.language | eng | - |
| dc.publisher | DGIST | - |
| dc.title | Molecular mechanism of voltage-gated Ca2+ channel regulation by membrane PIP2 | - |
| dc.title.alternative | 세포막 인지질 PIP2에 의한 전압 개폐 칼슘채널의 활성 조절 메커니즘 규명 | - |
| dc.type | Thesis | - |
| dc.identifier.doi | 10.22677/Theses.200000281554 | - |
| dc.description.degree | Doctor | - |
| dc.contributor.department | Brain and Cognitive Sciences | - |
| dc.contributor.coadvisor | Jang, Deok-Jin | - |
| dc.date.awarded | 2020-02 | - |
| dc.publisher.location | Daegu | - |
| dc.description.database | dCollection | - |
| dc.citation | XT.BD 박83 202002 | - |
| dc.date.accepted | 2020-01-20 | - |
| dc.contributor.alternativeDepartment | 뇌인지과학전공 | - |
| dc.embargo.liftdate | 2022-01-01 | - |
| dc.contributor.affiliatedAuthor | Suh, Byung-Chang | - |
| dc.contributor.affiliatedAuthor | Park, Cheon-Gyu | - |
| dc.contributor.affiliatedAuthor | Jang, Deok-Jin | - |
| dc.contributor.alternativeName | 박천규 | - |
| dc.contributor.alternativeName | 장덕진 | - |
| dc.contributor.alternativeName | Suh, Byung-Chang | - |
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