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Biodegradation of Polystyrene by Pseudomonas sp. Isolated from the Gut of Superworms (Larvae of Zophobas atratus)

Title
Biodegradation of Polystyrene by Pseudomonas sp. Isolated from the Gut of Superworms (Larvae of Zophobas atratus)
Authors
Kim, Hong RaeLee, Hyun MinYu, Hee CheolJeon, EunbeenLee, SukkyooLi, JiaojieKim, Dae-Hwan
DGIST Authors
Lee, Sukkyoo
Issue Date
2020-06
Citation
Environmental Science and Technology, 54(11), 6987-6996
Type
Article
Article Type
Article
Keywords
LOW-DENSITY POLYETHYLENEMICROBIAL-DEGRADATIONBIOFILM FORMATIONPLASTICSMINERALIZATIONPERSPECTIVESPOLYTHENEPRODUCTSSTRAINSSOIL
ISSN
0013-936X
Abstract
Recently, various attempts have been made to solve plastic waste problems, such as development of biodegradation without producing pollution. Polystyrene (PS) is the fifth most used plastic in many industries; therefore, degrading PS becomes a critical global issue. Here, we reported Pseudomonas aeruginosa strain DSM 50071, initially isolated from the gut of the superworms, Zophobas atratus, and the PS degradation by Pseudomonas sp. DSM 50071. We examined PS degradation using electronic microscopy and measured changes in atomic composition and contact angles with water droplets on the PS surface that represents a chemical change from hydrophobicity to hydrophilicity. We have further examined chemical structural changes using X-ray photoelectron spectroscopy, Fourier-transforminfrared spectroscopy, and nuclear magnetic resonance (NMR) to confirm the formation of carbonyl groups (C=O) in the oxidation pathway during PS biodegradation. In reverse transcription quantitative polymerase chain reaction analysis, the gene expression level of serine hydrolase (SH) in Pseudomonas sp. DSM 50071 was highly increased during PS degradation, and the enzyme-mediated biodegradation of PS was further confirmed by the SH inhibitor treatment test. Thus, the significance of these findings goes beyond the discovery of a novel function of Pseudomonas sp. DSM 50071 in the gut of superworms, highlighting a potential solution for PS biodegradation.
URI
http://hdl.handle.net/20.500.11750/12035
DOI
10.1021/acs.est.0c01495
Publisher
American Chemical Society
Related Researcher
  • Author Lee, Sukkyoo Laboratory of Environmental Biotechnology
  • Research Interests Biodegradation of Plastics; 플라스틱 생분해
Files:
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Collection:
Department of Brain and Cognitive SciencesLaboratory of Environmental Biotechnology1. Journal Articles


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