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Genomic and histopathological characteristics of Vibrio parahaemolyticus isolated from an acute hepatopancreatic necrosis disease outbreak in Pacific white shrimp (Penaeus vannamei) cultured in Korea

Title
Genomic and histopathological characteristics of Vibrio parahaemolyticus isolated from an acute hepatopancreatic necrosis disease outbreak in Pacific white shrimp (Penaeus vannamei) cultured in Korea
Authors
Han, Jee EunChoi, Seong-KyoonHan, Se-HyeonLee, Seung ChanJeon, Hye JinLee, ChorongKim, Kyeong YeonLee, Young SeoPark, Seul ChanRhee, GahngyoonPark, Seon YoungKim, Jun-SeobPark, SongKim, Ji HyungLee, Kyeong-Jun
DGIST Authors
Han, Jee Eun; Choi, Seong-Kyoon; Han, Se-Hyeon; Lee, Seung Chan; Jeon, Hye Jin; Lee, Chorong; Kim, Kyeong Yeon; Lee, Young Seo; Park, Seul Chan; Rhee, Gahngyoon; Park, Seon Young; Kim, Jun-Seob; Park, Song; Kim, Ji Hyung; Lee, Kyeong-Jun
Issue Date
2020-07
Citation
Aquaculture, 524, 735284
Type
Article
Article Type
Article
Author Keywords
Acute hepatopancreatic necrosis diseaseKoreaPenaeus vannameiVibrio parahaemolyticus
Keywords
CAUSATIVE AGENTLITOPENAEUS-VANNAMEIPLASMIDAHPNDVIRULENCETUMEFACIENSRESISTANCEPROTEINSTRAIN
ISSN
0044-8486
Abstract
This report describes the detailed microbial and histological characteristics of Vibrio parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (V-pAHPND) that infected Pacific white shrimp (Penaeus vannamei) cultured in Korea. Shrimps showing typical symptoms of AHPND, including a whitish hepatopancreas and empty gut, were reported at two private shrimp farms in Korea in 2019. In the hepatopancreas of the field samples, sloughing of undifferentiated cells in the hepatopancreatic tubule epithelium was observed upon histopathological examination, and 2 Vibrio spp.-related toxin genes (pirA and pirB) associated with AHPND were detected in the samples. Among samples obtained from the hepatopancreas, pond water, and feces (pond sediment), the copy number of the virulent AHPND plasmid was the highest in the hepatopancreas samples, followed by that in the pond water and feces (pond sediment) samples. Two V-pAHPND strains were isolated from pond water and diseased shrimp. The genome of the virulent AHPND plasmid pVp_Kor-D1-2 (GenBank accession no. CP046414.1), isolated from strain 19-021-D1, was fully sequenced. We confirmed that the Korean VpAHPND strain harbored the Asian-type AHPND plasmid, which was almost identical to the AHPND-associated V. owensii plasmid pVOWZ2 previously identified in China. Furthermore, the pathogenicity of the Korean VpAHPND strains was evaluated in laboratory bioassays, and we verified that those isolates were highly pathogenic to Pacific white shrimp, causing high cumulative mortality within 3 days.
URI
http://hdl.handle.net/20.500.11750/12055
DOI
10.1016/j.aquaculture.2020.735284
Publisher
Elsevier BV
Related Researcher
Files:
There are no files associated with this item.
Collection:
Division of Biotechnology1. Journal Articles


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