Cited 6 time in webofscience Cited 6 time in scopus

Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

Title
Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
Authors
Baek, Seung HeeSong, Hyun WooLee, SunwoongKim, Jung-EunKim, Yeo HyangWi, Jung-SubOk, Jong G.Park, Jun SeokHong, SeonkiKwak, Moon KyuLee, Hye JinNam, Sung-Wook
DGIST Authors
Hong, Seonki
Issue Date
2020-05
Citation
Frontiers in Chemistry, 8, 285
Type
Article
Article Type
Article
Author Keywords
Au nanocubeIL-10Au LSPR striproll-to-rollnanoarchitecture
Keywords
SURFACE-PLASMON RESONANCERANGE
ISSN
2296-2646
Abstract
Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanoparticle on the Au strip causes a red-shift of the LSPR extinction peak, enabling the detection of interleukin-10 (IL-10) cytokine. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprinting process to create nanograting structures on polyethylene terephthalate (PET) film. By the angled deposition of Au on the PET film, we demonstrated a double-bent Au structure with a strong LSPR extinction peak at ~760 nm. Using the Au LSPR sensor, we developed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure of IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced colloidal Au nanocube (AuNC) to be cross-linked with IL-10 detection antibody for immunogold assay. Using IL-10 as a model protein, we successfully achieved nanomolar sensitivity. We confirmed that the shift of the extinction peak was improved by 450% due to plasmon coupling between AuNC and Au strip. We expect that the AuNC-assisted LSPR sensor platform can be utilized as a diagnostic tool by providing convenient and fast detection of the LSPR signal. © Copyright © 2020 Baek, Song, Lee, Kim, Kim, Wi, Ok, Park, Hong, Kwak, Lee and Nam.
URI
http://hdl.handle.net/20.500.11750/12102
DOI
10.3389/fchem.2020.00285
Publisher
Frontiers Media S.A.
Related Researcher
  • Author Hong, Seonki Biomaterials & Biointerface Engineering Laboratory
  • Research Interests Bio-inspired organic materials; Polymeric biomaterials; Surface biofunctionalization; biochip fabrication
Files:
Collection:
Department of Emerging Materials ScienceBiomaterials & Biointerface Engineering Laboratory1. Journal Articles


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