Cited 6 time in
Cited 6 time in
Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
- Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10
- Baek, Seung Hee; Song, Hyun Woo; Lee, Sunwoong; Kim, Jung-Eun; Kim, Yeo Hyang; Wi, Jung-Sub; Ok, Jong G.; Park, Jun Seok; Hong, Seonki; Kwak, Moon Kyu; Lee, Hye Jin; Nam, Sung-Wook
- DGIST Authors
- Hong, Seonki
- Issue Date
- Frontiers in Chemistry, 8, 285
- Article Type
- Author Keywords
- Au nanocube; IL-10; Au LSPR strip; roll-to-roll; nanoarchitecture
- SURFACE-PLASMON RESONANCE; RANGE
- Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanoparticle on the Au strip causes a red-shift of the LSPR extinction peak, enabling the detection of interleukin-10 (IL-10) cytokine. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprinting process to create nanograting structures on polyethylene terephthalate (PET) film. By the angled deposition of Au on the PET film, we demonstrated a double-bent Au structure with a strong LSPR extinction peak at ~760 nm. Using the Au LSPR sensor, we developed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure of IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced colloidal Au nanocube (AuNC) to be cross-linked with IL-10 detection antibody for immunogold assay. Using IL-10 as a model protein, we successfully achieved nanomolar sensitivity. We confirmed that the shift of the extinction peak was improved by 450% due to plasmon coupling between AuNC and Au strip. We expect that the AuNC-assisted LSPR sensor platform can be utilized as a diagnostic tool by providing convenient and fast detection of the LSPR signal. © Copyright © 2020 Baek, Song, Lee, Kim, Kim, Wi, Ok, Park, Hong, Kwak, Lee and Nam.
- Frontiers Media S.A.
- Related Researcher
Biomaterials & Biointerface Engineering Laboratory
Bio-inspired organic materials; Polymeric biomaterials; Surface biofunctionalization; biochip fabrication
- Department of Emerging Materials ScienceBiomaterials & Biointerface Engineering Laboratory1. Journal Articles
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