Cited 0 time in webofscience Cited 0 time in scopus

Compact 256-channel multi-well microelectrode array system for in vitro neuropharmacology test

Title
Compact 256-channel multi-well microelectrode array system for in vitro neuropharmacology test
Authors
Kim, DaejeongKang, HongkiNam, Yoonkey
DGIST Authors
Kang, Hongki
Issue Date
2020-09
Citation
Lab on a Chip, 20(18), 3410-3422
Type
Article
Article Type
Article
Keywords
CORTICAL CULTURESMEA PLATFORMLARGE-SCALENEURONSEXCITOTOXICITYSURFACE
ISSN
1473-0197
Abstract
Microelectrode arrays (MEAs) have been extensively used to measure extracellular spike activity from cultured neurons using multiple electrodes embedded in a planar glass substrate. This system has been implemented to investigate drug effects by detecting pharmacological perturbation reflected in spontaneous network activity. By configuring multiple wells in an MEA, a high-throughput electrophysiological assay has become available, speeding up drug tests. Despite its merits in acquiring massive amounts of electrophysiological data, the high cost and the bulky size of commercial multi-well MEA systems and most importantly its lack of customizability prevent potential users from fully implementing the system in drug experiments. In this work, we have developed a microelectrode array based drug testing platform by incorporating a custom-made compact 256-channel multi-well MEA in a standard microscope slide and commercial application-specific integrated circuit (ASIC) chip based recording system. We arranged 256 electrodes in 16 wells to maximize data collection from a single chip. The multi-well MEA in this work has a more compact design with reduced chip size compared to previously reported multi-well MEAs. Four synaptic modulators (NMDA, AMPA, bicuculline (BIC) and ATP) were applied to a multi-well MEA and neural spike activity was analyzed to study their neurophysiological effects on cultured neurons. Analyzing various neuropharmacological compounds has become much more accessible by utilizing commercially available digital amplifier chips and customizing a user-preferred analog-front-end interface design with additional benefits in reduced platform size and cost.
URI
http://hdl.handle.net/20.500.11750/12414
DOI
10.1039/d0lc00384k
Publisher
Royal Society of Chemistry
Related Researcher
  • Author Kang, Hongki Advanced Electronic Devices Research Group(AEDRG) - Kang Lab.
  • Research Interests Bioelectronics; Neural interfaces; Flexible electronics; Printed electronics; 바이오전자; 신경 인터페이스; 뇌공학; 플렉서블 전자; 반도체 소자; 인쇄 전자
Files:
There are no files associated with this item.
Collection:
Department of Information and Communication EngineeringAdvanced Electronic Devices Research Group(AEDRG) - Kang Lab.1. Journal Articles


qrcode mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE