Cited 0 time in
Cited 0 time in
Compact 256-channel multi-well microelectrode array system for in vitro neuropharmacology test
- Compact 256-channel multi-well microelectrode array system for in vitro neuropharmacology test
- Kim, Daejeong; Kang, Hongki; Nam, Yoonkey
- DGIST Authors
- Kang, Hongki
- Issue Date
- Lab on a Chip, 20(18), 3410-3422
- Article Type
- CORTICAL CULTURES; MEA PLATFORM; LARGE-SCALE; NEURONS; EXCITOTOXICITY; SURFACE
- Microelectrode arrays (MEAs) have been extensively used to measure extracellular spike activity from cultured neurons using multiple electrodes embedded in a planar glass substrate. This system has been implemented to investigate drug effects by detecting pharmacological perturbation reflected in spontaneous network activity. By configuring multiple wells in an MEA, a high-throughput electrophysiological assay has become available, speeding up drug tests. Despite its merits in acquiring massive amounts of electrophysiological data, the high cost and the bulky size of commercial multi-well MEA systems and most importantly its lack of customizability prevent potential users from fully implementing the system in drug experiments. In this work, we have developed a microelectrode array based drug testing platform by incorporating a custom-made compact 256-channel multi-well MEA in a standard microscope slide and commercial application-specific integrated circuit (ASIC) chip based recording system. We arranged 256 electrodes in 16 wells to maximize data collection from a single chip. The multi-well MEA in this work has a more compact design with reduced chip size compared to previously reported multi-well MEAs. Four synaptic modulators (NMDA, AMPA, bicuculline (BIC) and ATP) were applied to a multi-well MEA and neural spike activity was analyzed to study their neurophysiological effects on cultured neurons. Analyzing various neuropharmacological compounds has become much more accessible by utilizing commercially available digital amplifier chips and customizing a user-preferred analog-front-end interface design with additional benefits in reduced platform size and cost.
- Royal Society of Chemistry
- Related Researcher
Advanced Electronic Devices Research Group(AEDRG) - Kang Lab.
Bioelectronics; Neural interfaces; Flexible electronics; Printed electronics; 바이오전자; 신경 인터페이스; 뇌공학; 플렉서블 전자; 반도체 소자; 인쇄 전자
There are no files associated with this item.
- Department of Information and Communication EngineeringAdvanced Electronic Devices Research Group(AEDRG) - Kang Lab.1. Journal Articles
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.