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Mass spectrometry-based proteomics of single cells and organoids: The new generation of cancer research
- Mass spectrometry-based proteomics of single cells and organoids: The new generation of cancer research
- Ntwali, P. V. Migisha; Heo, Chae Eun; Han, Jong Yoon; Chae, Soo Yeon; Kim, Minji; Vu, Minh Hung; Kim, Min-Sik; Kim, Hugh I.
- DGIST Authors
- Kim, Min-Sik
- Issue Date
- TrAC - Trends in Analytical Chemistry, 130
- Article Type
- Author Keywords
- Mass spectrometry (MS); Proteomics; Cellular heterogeneity; 3D cell; Single cell
- SAMPLE PREPARATION; LIQUID-CHROMATOGRAPHY; SPHEROID CULTURE; DRUG-RESISTANCE; TUMOR; DISCOVERY; IDENTIFICATION; TECHNOLOGIES; TANDEM; MODEL
- Mass spectrometry (MS)-based proteomics is becoming a widely used technique for proteome characterization. Remarkable improvements in the resolution and sensitivity of MS instruments have enabled MS to address an ever-increasing array of biological questions. Nevertheless, the intrinsic cellular heterogeneity of biological systems continues to be a hurdle in MS-based proteomics for cancer research. MS-based proteomics of 3D cellular models and single-cell systems are being used as an effective analysis technique to characterize cellular heterogeneity among cancer cells. Proteomic analysis of 3D cellular models and single-cell systems provides a correlation between cellular heterogeneity and patient-specific responses to chemotherapy drugs, leading to effective and less toxic cancer treatments. Here, we review the pioneering achievements in MS-based proteomic analysis of 3D cell models and single-cell systems. Additionally, we provide insights into the contribution of MS-based proteomics, from examining tumoroid and single-cell systems to the development of precision medicine for cancer treatment. © 2020 Elsevier B.V.
- Elsevier BV
- Related Researcher
Laboratory for QBIO and Precision Medicine
Cancer Proteogenomics, Biomarker Discovery, Integrative Multi-Omics
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- Department of New BiologyLaboratory for QBIO and Precision Medicine1. Journal Articles
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