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A Microfluidic System for Stable and Continuous EEG Monitoring from Multiple Larval Zebrafish

Title
A Microfluidic System for Stable and Continuous EEG Monitoring from Multiple Larval Zebrafish
Authors
Lee, YuhyunSeo, Hee WonLee, Kyeong JaeJang, Jae-WonKim, Sohee
DGIST Authors
Kim, Sohee
Issue Date
2020-10
Citation
Sensors, 20(20), 5903
Type
Article
Article Type
Article
Author Keywords
larval zebrafishelectroencephalogram (EEG)microfluidic channelagarose-freedrug screeninganti-epileptic drugs
Keywords
EPILEPTIC SEIZURESMODELIDENTIFYSCREENFISHTOOL
ISSN
1424-8220
Abstract
Along with the increasing popularity of larval zebrafish as an experimental animal in the fields of drug screening, neuroscience, genetics, and developmental biology, the need for tools to deal with multiple larvae has emerged. Microfluidic channels have been employed to handle multiple larvae simultaneously, even for sensing electroencephalogram (EEG). In this study, we developed a microfluidic chip capable of uniform and continuous drug infusion across all microfluidic channels during EEG recording. Owing to the modular design of the microfluidic channels, the number of animals under investigation can be easily increased. Using the optimized design of the microfluidic chip, liquids could be exchanged uniformly across all channels without physically affecting the larvae contained in the channels, which assured a stable environment maintained all the time during EEG recording, by eliminating environmental artifacts and leaving only biological effects to be seen. To demonstrate the usefulness of the developed system in drug screening, we continuously measured EEG from four larvae without and with pentylenetetrazole application, up to 60 min. In addition, we recorded EEG from valproic acid (VPA)-treated zebrafish and demonstrated the suppression of seizure by VPA. The developed microfluidic system could contribute to the mass screening of EEG for drug development to treat neurological disorders such as epilepsy in a short time, owing to its handy size, cheap fabrication cost, and the guaranteed uniform drug infusion across all channels with no environmentally induced artifacts. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
URI
http://hdl.handle.net/20.500.11750/12485
DOI
10.3390/s20205903
Publisher
MDPI AG
Related Researcher
  • Author Kim, Sohee Neural Interfaces & MicroSystems Lab
  • Research Interests Neural interface; Brain interface; Bio MEMS; Soft MEMS; Stretchable electronics; Zebrafish electrophysiology
Files:
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Collection:
Department of Robotics EngineeringNeural Interfaces & MicroSystems Lab1. Journal Articles


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