Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Hwang, Yeo Jin | - |
dc.contributor.author | Jung, Gwon-Soo | - |
dc.contributor.author | Jeon, Wonbae | - |
dc.contributor.author | Lee, Kyeong-Min | - |
dc.date.accessioned | 2020-12-06T13:13:10Z | - |
dc.date.available | 2020-12-06T13:13:10Z | - |
dc.date.created | 2020-09-24 | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 2405-8440 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/12517 | - |
dc.description.abstract | Objective: Enhancement of β-cell proliferation plays an important role in maintaining β-cell mass and function, and in improving pancreatic β-cell survival before transplantation. Extracellular matrix (ECM) components increase the adhesion and proliferation of β-cells, and the RGD-modified elastin-like polypeptide (RGD-ELP, REP) has been described as a bioactive matrix. In this study, we investigated whether REP could enhance β-cell adhesion and proliferation and elucidated the signaling pathways involved. Methods: We investigated the effect of REP on cell adhesion, proliferation and insulin secretion via assays using Rin-m and rat islets. Crystal violet, CCK-8, and BrdU assay, FACS, western blot, real time q-PCR analyses and insulin ELISA were examined. To explain the associated mechanisms, phosphorylation of Akt and extracellular signal-regulated kinase (Erk) were measured. Results: REP more increased the adhesion, proliferation and survival of Rin-m cells compared to elastin-like poly peptide (ELP) without RGD-motif. The enhancement of β-cell proliferation by REP was associated with increased cyclin D1, cyclin D2 and cdk6, and decreased p27 levels. When β-cells were cultured on REP, Erk and the phosphatidylinositol 3-kinase (PI3-kinase) downstream effector, Akt was stimulated. Treatment with the Erk pathway inhibitor and PI3-kinase inhibitor decreased REP-induced β-cell adhesion and proliferation, and regulated REP-induced cell cycle proteins. Additionally, REP increased the mRNA and protein levels of insulin and its transcription factor, PDX-1, and insulin secretion. Conclusions: Our results demonstrate that the up-regulation of the PI3K/Akt and Erk signaling pathways and the regulation of cell cycle proteins by REP could serve as effective strategies for improving pancreatic β-cell adhesion and proliferation. © 2020 The Author(s)Tissue engineering; Biomedical materials; Cell biology; Biotechnology; Diabetes; Insulin; |
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dc.language | English | - |
dc.publisher | Elsevier BV | - |
dc.title | Arg-Gly-Asp-modified elastin-like polypeptide regulates cell proliferation and cell cycle proteins via the phosphorylation of Erk and Akt in pancreatic β-cell. | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.heliyon.2020.e04918 | - |
dc.identifier.scopusid | 2-s2.0-85090695295 | - |
dc.identifier.bibliographicCitation | Heliyon, v.6, no.9, pp.e04918 | - |
dc.description.isOpenAccess | TRUE | - |
dc.subject.keywordAuthor | RGD-modified elastin-like polypeptide | - |
dc.subject.keywordAuthor | Tissue engineering | - |
dc.subject.keywordAuthor | β-cell adhesion | - |
dc.subject.keywordAuthor | β-cell proliferation | - |
dc.subject.keywordAuthor | Biomedical materials | - |
dc.subject.keywordAuthor | Biotechnology | - |
dc.subject.keywordAuthor | Cell biology | - |
dc.subject.keywordAuthor | Cell cycle | - |
dc.subject.keywordAuthor | Diabetes | - |
dc.subject.keywordAuthor | Insulin | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | BETA-CELLS | - |
dc.subject.keywordPlus | RGD PEPTIDES | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | ISLETS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.citation.number | 9 | - |
dc.citation.startPage | e04918 | - |
dc.citation.title | Heliyon | - |
dc.citation.volume | 6 | - |