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Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity

Title
Extracellular vesicles derived from macrophages display glycyl-tRNA synthetase 1 and exhibit anti-cancer activity
Authors
Goughnour, Peter C.Park, Min ChulKim, Sang BumJun, SangmiYang, Won SukChae, SehyunCho, SeongminSong, ChihongLee, Ji-HyunHyun, Jae KyungKim, Byung GyuHwang, DaeheeJung, Hyun SukGho, Yong SongKim, Sunghoon
DGIST Authors
Goughnour, Peter C.; Park, Min Chul; Kim, Sang Bum; Jun, Sangmi; Yang, Won Suk; Chae, Sehyun; Cho, Seongmin; Song, Chihong; Lee, Ji-Hyun; Hyun, Jae Kyung; Kim, Byung Gyu; Hwang, Daehee; Jung, Hyun Suk; Gho, Yong Song; Kim, Sunghoon
Issue Date
2020-11
Citation
Journal of Extracellular Vesicles, 10(1), e12029
Type
Article
Article Type
Article
Author Keywords
extracellular vesiclesglycyl-tRNA synthetase 1macrophagepalmitoylationtumour microenvironment
ISSN
2001-3078
Abstract
Glycyl-tRNA synthetase 1 (GARS1), a cytosolic enzyme secreted from macrophages, promotes apoptosis in cancer cells. However, the mechanism underlying GARS1 secretion has not been elucidated. Here, we report that GARS1 is secreted through unique extracellular vesicles (EVs) with a hydrodynamic diameter of 20–58 nm (mean diameter: 36.9 nm) and a buoyant density of 1.13–1.17 g/ml. GARS1 was anchored to the surface of these EVs through palmitoylated C390 residue. Proteomic analysis identified 164 proteins that were uniquely enriched in the GARS1-containing EVs (GARS1-EVs). Among the identified factors, insulin-like growth factor II receptor, and vimentin also contributed to the anti-cancer activity of GARS1-EVs. This study identified the unique secretory vesicles containing GARS1 and various intracellular factors that are involved in the immunological defence response against tumorigenesis. © 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles
URI
http://hdl.handle.net/20.500.11750/12978
DOI
10.1002/jev2.12029
Publisher
John Wiley and Sons Inc
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