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Suppressing background noise in STED optical nanoscopy

Title
Suppressing background noise in STED optical nanoscopy
Authors
Jeong, SejooKim, JaeyongLee, Jong-Chan
DGIST Authors
Jeong, Sejoo; Kim, Jaeyong; Lee, Jong-Chan
Issue Date
2021-03
Citation
Journal of the Korean Physical Society, 78(5), 401-407
Type
Article
Article Type
Review
Author Keywords
Super-resolution microscopySTEDBackground noise
Keywords
EMISSION DEPLETION MICROSCOPYSTIMULATED-EMISSIONRESOLUTIONSUBTRACTION
ISSN
0374-4884
Abstract
Super-resolution microscopy or optical nanoscopy has enabled all-optical observation of nanoscopic objects with resolution beyond Abbe’s diffraction limit. STimulated Emission Depletion (STED) nanoscopy achieves diffraction-unlimited resolution by employing a de-excitation laser with a hollow core in addition to the conventional excitation laser in confocal microscopy. One of the strong unexpected adverse effects in resolution improvement in STED nanoscopy is a structured, systematic background noise, which deteriorates the desired super-resolved image. Suppressing background noise, therefore, has been an important challenge in STED nanoscopy. Here, we introduce the characteristics of the background noise in STED nanoscopy and review several recent approaches that tackle this problem. The methods will be examined in comparison to one another and the advantages and disadvantages of each method will be highlighted. © 2021, The Korean Physical Society.
URI
http://hdl.handle.net/20.500.11750/12984
DOI
10.1007/s40042-021-00057-7
Publisher
한국물리학회
Related Researcher
  • Author Lee, Jong-Chan Single-molecule Biophysics and Advanced Bioimaging Laboratory
  • Research Interests Liquid-liquid phase separation; Super-resolution imaging;
Files:
There are no files associated with this item.
Collection:
Department of New BiologySingle-molecule Biophysics and Advanced Bioimaging Laboratory1. Journal Articles


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