Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Ham, Donghee | - |
dc.contributor.author | Ahn, Donghoon | - |
dc.contributor.author | Ashim, Janbolat | - |
dc.contributor.author | Cho, Yejin | - |
dc.contributor.author | Kim, Hee Ryung | - |
dc.contributor.author | Yu, Wookyung | - |
dc.contributor.author | Chung, Ka Young | - |
dc.date.accessioned | 2021-04-29T12:30:32Z | - |
dc.date.available | 2021-04-29T12:30:32Z | - |
dc.date.created | 2021-01-28 | - |
dc.date.issued | 2021-03 | - |
dc.identifier.issn | 1047-8477 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/13479 | - |
dc.description.abstract | Heterotrimeric guanine nucleotide-binding proteins (G proteins) are composed of α, β, and γ subunits. Gα switches between guanosine diphosphate (GDP)-bound inactive and guanosine triphosphate (GTP)-bound active states, and Gβγ interacts with the GDP-bound state. The GDP-binding regions are composed of two sites: the phosphate-binding and guanine-binding regions. The turnover of GDP and GTP is induced by guanine nucleotide-exchange factors (GEFs), including G protein-coupled receptors (GPCRs), Ric8A, and GIV/Girdin. However, the key structural factors for stabilizing the GDP-bound state of G proteins and the direct structural event for GDP release remain unclear. In this study, we investigated structural factors affecting GDP release by introducing point mutations in selected, conserved residues in Gαi3. We examined the effects of these mutations on the GDP/GTP turnover rate and the overall conformation of Gαi3 as well as the binding free energy between Gαi3 and GDP. We found that dynamic changes in the phosphate-binding regions are an immediate factor for the release of GDP. © 2021 Elsevier Inc. | - |
dc.language | English | - |
dc.publisher | Academic Press | - |
dc.title | Conformational switch that induces GDP release from Gi | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jsb.2020.107694 | - |
dc.identifier.wosid | 000629940300020 | - |
dc.identifier.scopusid | 2-s2.0-85099447380 | - |
dc.identifier.bibliographicCitation | Journal of Structural Biology, v.213, no.1, pp.107694 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordAuthor | Heterotrimeric guanine nucleotide-binding proteins | - |
dc.subject.keywordAuthor | GDP release | - |
dc.subject.keywordAuthor | Structure | - |
dc.subject.keywordPlus | protein structure | - |
dc.subject.keywordPlus | Galphai1 protein | - |
dc.subject.keywordPlus | Galphai2 protein | - |
dc.subject.keywordPlus | Galphai3 protein | - |
dc.subject.keywordPlus | Galphai4 protein | - |
dc.subject.keywordPlus | Galphai5 protein | - |
dc.subject.keywordPlus | Gbeta6 protein | - |
dc.subject.keywordPlus | guanosine diphosphate | - |
dc.subject.keywordPlus | guanosine triphosphate | - |
dc.subject.keywordPlus | inhibitory guanine nucleotide binding protein | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | carboxy terminal sequence | - |
dc.subject.keywordPlus | conformational transition | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | molecular dynamics | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | point mutation | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | protein binding | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein function | - |
dc.subject.keywordPlus | protein protein interaction | - |
dc.subject.keywordPlus | protein secretion | - |
dc.subject.keywordPlus | protein purification | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 107694 | - |
dc.citation.title | Journal of Structural Biology | - |
dc.citation.volume | 213 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Biophysics; Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology; Biophysics; Cell Biology | - |
dc.type.docType | Article | - |
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