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Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Phospholipid Changes in a Drosophila Model of Early Amyotrophic Lateral Sclerosis

Title
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Phospholipid Changes in a Drosophila Model of Early Amyotrophic Lateral Sclerosis
Authors
Jang, Hyun JunLe, Minh Uyen ThiPark, Jeong HyangChung, Chang GeonShon, Jin GyeongLee, Ga SeulMoon, Jeong HeeLee, Sung BaeChoi, Joon SigLee, Tae GeolYoon, Sohee
DGIST Authors
Jang, Hyun Jun; Le, Minh Uyen Thi; Park, Jeong Hyang; Chung, Chang Geon; Shon, Jin Gyeong; Lee, Ga Seul; Moon, Jeong Hee; Lee, Sung Bae; Choi, Joon Sig; Lee, Tae Geol; Yoon, Sohee
Issue Date
2021-08
Citation
Journal of the American Society for Mass Spectrometry, 32(10), 2536-2545
Type
Article
Author Keywords
amyotrophic lateral sclerosisDrosophila brainMALDImass spectrometry imagingphospholipids
ISSN
1044-0305
Abstract
Amyotrophic lateral sclerosis (ALS) is a degenerative disease caused by motor neuron damage in the central nervous system, and it is difficult to diagnose early. Drosophila melanogaster is widely used to investigate disease mechanisms and discover biomarkers because it is easy to induce disease in Drosophila through genetic engineering. We performed matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to investigate changes in phospholipid distribution in the brain tissue of an ALS-induced Drosophila model. Fly brain tissues of several hundred micrometers or less were sampled using a fly collar to obtain reproducible tissue sections of similar sizes. MSI of brain tissues of Drosophila cultured for 1 or 10 days showed that the distribution of phospholipids, including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylserine (PS), and phosphatidylinositol (PI), was significantly different between the control group and the ALS group. In addition, the lipid profile according to phospholipids differed as the culture time increased from 1 to 10 days. These results suggest that disease indicators based on lipid metabolites can be discovered by performing MALDI-MSI on very small brain tissue samples from the Drosophila disease model to ultimately assess the phospholipid changes that occur in early-stage ALS. © 2021 American Society for Mass Spectrometry. Published by American Chemical Society. All rights reserved.
URI
http://hdl.handle.net/20.500.11750/15616
DOI
10.1021/jasms.1c00167
Publisher
American Chemical Society
Related Researcher
  • Author Lee, Sung Bae Laboratory of Neurodegenerative Diseases and Aging
  • Research Interests Cellular mechanism of neurodegenerative diseases; Neuronal maintenance and remodeling; 퇴행성 뇌질환의 세포기전; 신경계 유지 및 리모델링 연구
Files:
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Collection:
Department of Brain SciencesLaboratory of Neurodegenerative Diseases and Aging1. Journal Articles


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