Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Supianto, Mulya | - |
dc.contributor.author | Lee, Sang Hyuk | - |
dc.contributor.author | Jhung, Sung Hwa | - |
dc.contributor.author | Mohammad Hazara Begum | - |
dc.contributor.author | Minh Hung Vu | - |
dc.contributor.author | Kim, Min-Sik | - |
dc.contributor.author | Song, Woo-Young | - |
dc.contributor.author | Kim, Tae-Young | - |
dc.contributor.author | Lee, Hye Jin | - |
dc.date.accessioned | 2021-11-12T07:30:02Z | - |
dc.date.available | 2021-11-12T07:30:02Z | - |
dc.date.created | 2021-11-04 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/15794 | - |
dc.description.abstract | A fluorescent paper strip immunoassay in conjunction with carbon nanodots@silica (CND@SiO2) as a label was developed for the quantitative measurements of human serum amyloid A1 (hSAA1) in serum at clinically significant concentrations for lung cancer diagnosis. Monodispersed CND@SiO2 was prepared by cohydrolysis between silane-crosslinked carbon nanodots and silica precursors via the Ströber method and further attached covalently to anti-hSAA1 (14F8) monoclonal antibody [anti-hSAA1(14F8)] specific to the hSAA1 target. The hSAA1 concentrations were then determined by quantifying the blue fluorescence intensity upon 365nm excitation of the captured hSAA1 with anti-hSAA1(14F8)-CND@SiO2 conjugates in the test line on a paper strip where anti-hSAA1 (10G1) monoclonal antibody was physisorbed. The developed fluorescent paper strip with CND@SiO2 can detect hSAA1 at concentrations ranging from 0.1 to 5nM (R2 = 0.995), with a limit of detection of 0.258nM in 10mM phosphate buffer pH 7.4 containing human serum albumin. Theperformance of recovery (90.98–109.17%) and repeatability (coefficients of variation < 8.46%) obtained was also acceptable for quantitative determinations. Theplatform was employed for direct determinationof hSAA1 concentrations in undiluted serum samples from lung cancer patients (relative standard deviation (RSD) < 7.46%) and healthyhumans (RSD < 3.96%). The results were compared with those obtained using a commercially available enzyme-linked immunosorbent assay alongside liquid chromatography with tandem mass spectrometry measurements. Graphical abstract: [Figure not available: see fulltext.]. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. | - |
dc.language | English | - |
dc.publisher | Springer Verlag | - |
dc.title | Fluorescent paper strip immunoassay with carbon nanodots@silica for determination of human serum amyloid A1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00604-021-05019-1 | - |
dc.identifier.scopusid | 2-s2.0-85117617184 | - |
dc.identifier.bibliographicCitation | Microchimica Acta, v.188, no.11 | - |
dc.description.isOpenAccess | FALSE | - |
dc.subject.keywordAuthor | Carbon nanodots@silica | - |
dc.subject.keywordAuthor | Fluorescent paper strip | - |
dc.subject.keywordAuthor | Human serum amyloid A1 | - |
dc.subject.keywordAuthor | Lung cancer | - |
dc.subject.keywordAuthor | Serum analysis | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | PROTEIN CORONA | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | VALIDATION | - |
dc.citation.number | 11 | - |
dc.citation.title | Microchimica Acta | - |
dc.citation.volume | 188 | - |
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