Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Keum, Minho | - |
dc.contributor.author | Ito, Dai | - |
dc.contributor.author | Kim, Mi-Seong | - |
dc.contributor.author | Lin, Yuxi | - |
dc.contributor.author | Yoon, Kyeong-Hyeon | - |
dc.contributor.author | Kim, Jihoon | - |
dc.contributor.author | Lee, Sung-Hee | - |
dc.contributor.author | Kim, Ji-Hun | - |
dc.contributor.author | Yu, Wookyung | - |
dc.contributor.author | Lee, Young-Ho | - |
dc.contributor.author | Won, Hyung-Sik | - |
dc.date.accessioned | 2021-12-08T13:00:11Z | - |
dc.date.available | 2021-12-08T13:00:11Z | - |
dc.date.created | 2021-12-06 | - |
dc.date.issued | 2021-11 | - |
dc.identifier.citation | Biology, v.10, no.11 | - |
dc.identifier.issn | 2079-7737 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11750/15924 | - |
dc.description.abstract | Hsp33, a prokaryotic redox-regulated holding chaperone, has been recently identified to be able to exhibit an unfoldase and aggregase activity against elongation factor Tu (EF-Tu) in its reduced state. In this study, we investigated the effect of elongation factor Ts (EF-Ts) and trigger factor (TF) on Hsp33-mediated EF-Tu unfolding and aggregation using gel filtration, light scattering, circular dichroism, and isothermal titration calorimetry. We found that EF-Tu unfolding and subsequent aggregation induced by Hsp33 were evident even in its complex state with EF-Ts, which enhanced EF-Tu stability. In addition, although TF alone had no substantial effect on the stability of EF-Tu, it markedly amplified the Hsp33-mediated EF-Tu unfolding and aggregation. Collectively, the present results constitute the first example of synergistic unfoldase/aggregase activity of molecular chaperones and suggest that the stability of EF-Tu is modulated by a sophisticated network of molecular chaperones to regulate protein biosynthesis in cells under stress conditions. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | English | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Molecular Effects of Elongation Factor Ts and Trigger Factor on the Unfolding and Aggregation of Elongation Factor Tu Induced by the Prokaryotic Molecular Chaperone Hsp33 | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/biology10111171 | - |
dc.identifier.wosid | 000724147900001 | - |
dc.identifier.scopusid | 2-s2.0-85121853416 | - |
dc.type.local | Article(Overseas) | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.citation.publicationname | Biology | - |
dc.contributor.nonIdAuthor | Keum, Minho | - |
dc.contributor.nonIdAuthor | Ito, Dai | - |
dc.contributor.nonIdAuthor | Kim, Mi-Seong | - |
dc.contributor.nonIdAuthor | Lin, Yuxi | - |
dc.contributor.nonIdAuthor | Yoon, Kyeong-Hyeon | - |
dc.contributor.nonIdAuthor | Kim, Jihoon | - |
dc.contributor.nonIdAuthor | Lee, Sung-Hee | - |
dc.contributor.nonIdAuthor | Kim, Ji-Hun | - |
dc.contributor.nonIdAuthor | Lee, Young-Ho | - |
dc.contributor.nonIdAuthor | Won, Hyung-Sik | - |
dc.identifier.citationVolume | 10 | - |
dc.identifier.citationNumber | 11 | - |
dc.identifier.citationTitle | Biology | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordAuthor | aggregase activity | - |
dc.subject.keywordAuthor | EF-Tu | - |
dc.subject.keywordAuthor | EF-Ts | - |
dc.subject.keywordAuthor | proteostasis | - |
dc.subject.keywordAuthor | Hsp33 | - |
dc.subject.keywordAuthor | molecular chaperone | - |
dc.subject.keywordAuthor | protein biosynthesis | - |
dc.subject.keywordAuthor | trigger factor | - |
dc.subject.keywordAuthor | unfoldase activity | - |
dc.subject.keywordPlus | REDOX-REGULATED CHAPERONE | - |
dc.subject.keywordPlus | SWITCH DOMAIN | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | DNAK | - |
dc.contributor.affiliatedAuthor | Keum, Minho | - |
dc.contributor.affiliatedAuthor | Ito, Dai | - |
dc.contributor.affiliatedAuthor | Kim, Mi-Seong | - |
dc.contributor.affiliatedAuthor | Lin, Yuxi | - |
dc.contributor.affiliatedAuthor | Yoon, Kyeong-Hyeon | - |
dc.contributor.affiliatedAuthor | Kim, Jihoon | - |
dc.contributor.affiliatedAuthor | Lee, Sung-Hee | - |
dc.contributor.affiliatedAuthor | Kim, Ji-Hun | - |
dc.contributor.affiliatedAuthor | Yu, Wookyung | - |
dc.contributor.affiliatedAuthor | Lee, Young-Ho | - |
dc.contributor.affiliatedAuthor | Won, Hyung-Sik | - |
There are no files associated with this item.