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Development of a GFRAL antagonistic antibody for the amelioration of cancer cachexia

Title
Development of a GFRAL antagonistic antibody for the amelioration of cancer cachexia
Translated Title
암 악액질 증상 개선을 위한 GFRAL 길항 항체 개발
Authors
Beom Yong Lee
DGIST Authors
Beom Yong Lee; Kyungmoo YeaByung-Hoon Lee
Advisor(s)
예경무
Co-Advisor(s)
Byung-Hoon Lee
Issue Date
2022
Available Date
2022-03-08
Degree Date
2022/02
Type
Thesis
Keywords
Cancer cachexia, cisplatin, GDF15, GFRAL, antibody
Description
Cancer cachexia, cisplatin, GDF15, GFRAL, antibody
Table Of Contents
I. Introduction 1 1.1 Chemotherapy-induced cachexia 1 1.2 Growth differentiation factor 15 (GDF15) 1 1.3 GDF15/GFRAL/RET axis 2 1.4 Aims and objectives 2 II. Materials & Methods 4 2.1 Biopanning 4 2.2 Enzyme-linked immunosorbent assay (ELISA) 4 2.3 Antibody purification 5 2.4 Surface plasmon resonance (SPR) spectrometry 5 2.5 Cells and cell culture 5 2.6 Luciferase reporter assay 6 2.7 Flow cytometry analysis 6 2.8 Immunofluorescence staining 7 2.9 Western blot analysis 7 2.10 Animals and in vivo experiments 8 2.11 Statistical analysis 8 III. Results 9 3.1 GFRAL antagonist antibody was selected by biopanning using human combinatorial antibody phage libraries 9 3.2 Antibody A11 binds to GFRAL and potently suppresses the GDF15/GFRAL/RET axis 12 3.3 Antibody A11 attenuates cisplatin-induced cachexia through inhibition of the GDF15/GFRAL/RET axis in vivo 15 3.4 Antibody A11 alleviates cisplatin-induced cachexia in melanoma-bearing mice 19 IV. Discussion 24 V. References 26 VI. Summary 28
URI
http://dgist.dcollection.net/common/orgView/200000593969
http://hdl.handle.net/20.500.11750/16271
DOI
10.22677/thesis.200000593969
Degree
Master
Department
New Biology
University
DGIST
Related Researcher
  • Author Lee, Byung-Hoon Lab of Protein Homeostasis and Drug Discovery
  • Research Interests Ubiquitin-proteasome system; Protein homeostasis; Small-molecule chemical screening and drug discovery in human disease
Files:
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Collection:
Department of New BiologyThesesMaster


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