Title

Development of a GFRAL antagonistic antibody for the amelioration of cancer cachexia

Alternative Title

암 악액질 증상 개선을 위한 GFRAL 길항 항체 개발

DGIST Authors

Beom Yong Lee ; Kyungmoo Yea ; Byung-Hoon Lee

Advisor

예경무

Co-Advisor(s)

Byung-Hoon Lee

Issued Date

2022

Awarded Date

2022/02

Citation

Beom Yong Lee. (2022). Development of a GFRAL antagonistic antibody for the amelioration of cancer cachexia. doi: 10.22677/thesis.200000593969

Type

Thesis

Subject

Cancer cachexia, cisplatin, GDF15, GFRAL, antibody

Description

Cancer cachexia, cisplatin, GDF15, GFRAL, antibody

Table Of Contents

I. Introduction 1
1.1 Chemotherapy-induced cachexia 1
1.2 Growth differentiation factor 15 (GDF15) 1
1.3 GDF15/GFRAL/RET axis 2
1.4 Aims and objectives 2
II. Materials & Methods 4
2.1 Biopanning 4
2.2 Enzyme-linked immunosorbent assay (ELISA) 4
2.3 Antibody purification 5
2.4 Surface plasmon resonance (SPR) spectrometry 5
2.5 Cells and cell culture 5
2.6 Luciferase reporter assay 6
2.7 Flow cytometry analysis 6
2.8 Immunofluorescence staining 7
2.9 Western blot analysis 7
2.10 Animals and in vivo experiments 8
2.11 Statistical analysis 8
III. Results 9
3.1 GFRAL antagonist antibody was selected by biopanning using human combinatorial antibody phage libraries 9
3.2 Antibody A11 binds to GFRAL and potently suppresses the GDF15/GFRAL/RET axis 12
3.3 Antibody A11 attenuates cisplatin-induced cachexia through inhibition of the GDF15/GFRAL/RET axis in vivo 15
3.4 Antibody A11 alleviates cisplatin-induced cachexia in melanoma-bearing mice 19
IV. Discussion 24
V. References 26
VI. Summary 28

URI

http://dgist.dcollection.net/common/orgView/200000593969
http://hdl.handle.net/20.500.11750/16271

DOI

10.22677/thesis.200000593969

Degree

Master

Department

New Biology

Publisher

DGIST

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